Evidence map›Paper›PMID 41350304›Full record

ArticleScientific reports2025

Impact of dietary essential fatty acids on phospholipid composition and mitochondrial function in aged mares.

Kyle Fresa, Giovana D Catandi, Raul Gonzalez-Castro, Asma Omar, Luke A Whitcomb, Ming-Hao Cheng, Thomas W Chen, Elaine M Carnevale, Adam J Chicco

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Kyle FresaDepartment of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523-1680, USA.
Giovana D CatandiDepartment of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523-1680, USA.
Raul Gonzalez-CastroDepartment of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523-1680, USA.
Asma OmarDepartment of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523-1680, USA.
Luke A WhitcombDepartment of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523-1680, USA.
Ming-Hao ChengDepartment of Electrical and Computer Engineering, Colorado State University, Fort Collins, CO, 80523, USA.
Thomas W ChenDepartment of Electrical and Computer Engineering, Colorado State University, Fort Collins, CO, 80523, USA.
Elaine M CarnevaleDepartment of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523-1680, USA.
Adam J ChiccoDepartment of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523-1680, USA. Adam.chicco@colostate.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Advancing age is associated with a decline in fertility and functional capacity, which may result in part from suboptimal nutrition and impaired mitochondrial function. Dietary essential polyunsaturated fatty acids (PUFA) are broadly recommended to mitigate weight loss and reduce risk of chronic disease in aged populations, but their effects on mitochondrial function are less clear. The present study investigated the impacts of dietary supplementation with essential omega-3 PUFA (flaxseed oil; N3) or omega-6 PUFA (corn oil, N6) on blood, muscle and follicular cell fatty acid composition and mitochondrial function in aged light-horse mares (23.2 ± 1.1 year), which are excellent models of human reproductive aging and a focus of dietary interventions in the equine industry. Diets were fed for 6 weeks before adding a supplemental supportive nutrient formulation (DS) designed to optimize equine metabolic health for another 6 weeks. Results demonstrate tissue-specific effects of the dietary oils on mitochondrial function, most notably a decrease in oxidative capacity of platelets and muscle, and greater release of reactive oxygen species (ROS) from granulosa cells and muscle, with no significant difference in effects between N3 and N6 diets. Addition of the DS improved oxidative phosphorylation efficiency and tended to reduce ROS release across all cell types, and increased basal oocyte oxygen consumption. Taken together, these results provide novel insight to the diverse impacts of dietary PUFA intake on equine mitochondrial function, and suggest that supportive nutrients may be required to prevent negative health effects of dietary oil supplementation in aged mares.

Indexed as

AgingFatty Acids, EssentialMitochondriaPhospholipidsAnimal FeedAnimalsDietDietary SupplementsFatty Acids, Omega-3Fatty Acids, Omega-6FemaleHorsesReactive Oxygen SpeciesFatty Acids, EssentialFatty Acids, Omega-3Fatty Acids, Omega-6PhospholipidsReactive Oxygen SpeciesDietary supplementsMitochondrial functionPolyunsaturated fatty acidsReproductive health

Identifiers

PMID41350304
PMCPMC12686463

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.