Synergistic co-culture of Desertifilum tharense MASKD1 and bacterial consortium DBPS1 in an integrated biorefinery for sewage remediation and recovery of phycocyanin, biodiesel and green hydrogen.

Prasad Andhare, Shrey Dube, Bhargavi Sonavane, Pratik M Pataniya, C K Sumesh, Chirayu Desai, Bhavtosh Kikani, Datta Madamwar

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Article in World journal of microbiology & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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8 authors.

Prasad Andhare *Department of Biological Sciences, P. D. Patel Institute of Applied Sciences, Charotar University of Science and Technology, CHARUSAT campus, Dist. Anand, Changa, Gujarat, 388 412, India.

Shrey Dube *Department of Biological Sciences, P. D. Patel Institute of Applied Sciences, Charotar University of Science and Technology, CHARUSAT campus, Dist. Anand, Changa, Gujarat, 388 412, India.

Bhargavi Sonavane *Department of Biological Sciences, P. D. Patel Institute of Applied Sciences, Charotar University of Science and Technology, CHARUSAT campus, Dist. Anand, Changa, Gujarat, 388 412, India.

Pratik M PataniyaDepartment of Physical Sciences, P. D. Patel Institute of Applied Sciences, Charotar University of Science and Technology, CHARUSAT campus, Changa, Gujarat, 388 421, India.

C K SumeshDepartment of Physical Sciences, P. D. Patel Institute of Applied Sciences, Charotar University of Science and Technology, CHARUSAT campus, Changa, Gujarat, 388 421, India.

Chirayu DesaiDepartment of Environmental Biotechnology, Gujarat Biotechnology University, Gujarat International Finance Tec (GIFT)-City, Gandhinagar, Gujarat, 382355, India.

Bhavtosh KikaniDepartment of Biological Sciences, P. D. Patel Institute of Applied Sciences, Charotar University of Science and Technology, CHARUSAT campus, Dist. Anand, Changa, Gujarat, 388 412, India. bhavtoshkikani.bio@charusat.ac.in.ORCID http://orcid.org/0000-0003-1305-6225

Datta MadamwarDepartment of Biological Sciences, P. D. Patel Institute of Applied Sciences, Charotar University of Science and Technology, CHARUSAT campus, Dist. Anand, Changa, Gujarat, 388 412, India.ORCID http://orcid.org/0000-0003-3301-1120

Funding

Gujarat State Biotechnology Mission (GSBTM), Department of Science and Technology (DST), Government of Gujarat, India GSBTM/JD(R&D)/626/22-23/00018350

6 · The paper itself

Abstract

The present study proposes an integrated approach using a novel cyanobacterial-bacterial co-culture consisting of Desertifilum tharense MASKD1 and bacterial consortium DBPS1 to treat secondary sewage effluent in a raceway pond (50 L). Noticeably, the synergistic growth was achieved in the undiluted secondary sewage effluent without supplementation of any nutrients. The cyanobacterial-bacterial co-culture system achieved an improved tertiary sewage treatment as compared to the use of individual cultures. The Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) analysis confirmed an efficient removal of multiple ECs. The 16S rRNA gene amplicon sequencing explained how the co-culture community dynamics might have contributed in the bioremediation process. The harvested biomass was further valorized for the recovery of phycocyanin by ammonium sulphate fractionation followed by size exclusion chromatography. The antioxidant activity of phycocyanin was evaluated by Caenorhabditis elegans lifespan assays. The median survival duration of C. elegans was 14 days, which increased 1.64 times (to 23 days) in the presence of phycocyanin. In addition, the catalase activity assay in C. elegans further supported this observation. The catalase activity decreased by 20 Units/mg in worms treated with H

Indexed as

BiofuelsCyanobacteriaHydrogenMicrobial ConsortiaPhycocyaninSewageAnimalsAntioxidantsBacteriaBiodegradation, EnvironmentalBiomassCaenorhabditis elegansCoculture TechniquesRNA, Ribosomal, 16SAntioxidantsBiofuelsHydrogenPhycocyaninRNA, Ribosomal, 16SSewageBiodieselCaenorhabditis elegansCyanobacteriaEmerging contaminantsGreen hydrogenPhycocyanin

Identifiers

PMID41348244

DOI10.1007/s11274-025-04696-x

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