Evidence map›Paper›PMID 41348167›Full record

ReviewAntonie van Leeuwenhoek2025

In silico analysis of VP2 protein from infectious bursal disease virus isolate UPM1432/2019: structural dynamics and epitope prediction.

Ali Youssif Mansour, Abdul Rahman Omar, Mohd Hair Bejo, Noorjahan Banu Alitheen, Nurulfiza Mat Isa

Abstract readReview
PubMed Publisher
In one paragraph

Review in Antonie van Leeuwenhoek, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ali Youssif MansourDepartment of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Abdul Rahman OmarDepartment of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Mohd Hair BejoDepartment of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Noorjahan Banu AlitheenDepartment of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Nurulfiza Mat IsaDepartment of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia. nurulfiza@upm.edu.my.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Infectious Bursal Disease Virus (IBDV) remains a significant threat to global poultry health due to its immunosuppressive effects and high mutation rate, which challenge existing vaccine strategies. This study presents a comprehensive computational analysis of the VP2 protein from Malaysian IBDV isolate UPM1432/2019 to elucidate its structural stability and antigenic potential. Phylogenetic analysis revealed evolutionary divergence among VP2 sequences, while physicochemical profiling demonstrated the protein's high thermostability (aliphatic index: 98.77) and cellular stability (instability index: 17.55). Molecular dynamics simulations at 20 °C, 40 °C, 60 °C, and 80 °C showed progressive destabilization at elevated temperatures, indicated by increased RMSD, reduced hydrogen bonding, and higher solvent accessibility. Epitope prediction identified twelve linear and seven conformational B-cell epitopes, with several conserved, surface-exposed regions demonstrating high antigenicity. The most immunodominant conformational epitope (L168-E181) achieved a high ElliPro score of 0.903, indicating strong immune recognition potential. These findings highlight structurally stable and immunoreactive domains within VP2, offering valuable insights for the development of next-generation, thermostable epitope-based vaccines for effective IBDV control.

Indexed as

EpitopesInfectious bursal disease virusViral Structural ProteinsAmino Acid SequenceAnimalsBirnaviridae InfectionsComputer SimulationEpitopes, B-LymphocyteMolecular Dynamics SimulationPhylogenyPoultry DiseasesProtein ConformationEpitopesEpitopes, B-LymphocyteViral Structural ProteinsVP2 protein, infectious bursal disease virusAntigenicityEpitope predictionMolecular dynamics simulationProtein stabilityVP2 protein

Identifiers

PMID41348167

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.