Evidence map›Paper›PMID 41345359›Full record

ArticleJournal of integrative plant biology2026

Interspecies rice versus Arabidopsis thaliana protein-protein interactome profiling by touch-down overlapping PCR coupled with HiFi long-read sequencing.

Jie Huang, Yu Cheng, Jing Ruan, Xixi Liu, Dandan Xia, Yiting Chen, Delong Fan, Jiezheng Ying, Yifeng Wang, Xiaohong Tong and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of integrative plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Identification of CsAFP3-like as a novel gene regulating determinate growth of lateral branch in cucumber.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Jie HuangState Key Lab of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, 311400, China.ORCID https://orcid.org/0000-0002-2613-212X
Yu ChengState Key Lab of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, 311400, China.ORCID https://orcid.org/0009-0002-3303-9342
Jing RuanState Key Lab of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, 311400, China.ORCID https://orcid.org/0009-0003-4028-5565
Xixi LiuState Key Lab of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, 311400, China.ORCID https://orcid.org/0000-0003-3671-6054
Dandan XiaNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China.ORCID https://orcid.org/0009-0008-5576-1612
Yiting ChenState Key Lab of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, 311400, China.ORCID https://orcid.org/0009-0006-4429-927X
Delong FanState Key Lab of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, 311400, China.ORCID https://orcid.org/0009-0000-9319-1958
Jiezheng YingState Key Lab of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, 311400, China.ORCID https://orcid.org/0000-0001-8457-8405
Yifeng WangState Key Lab of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, 311400, China.ORCID https://orcid.org/0000-0002-5187-3755
Xiaohong TongState Key Lab of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, 311400, China.ORCID https://orcid.org/0000-0002-6580-5358
Zhiyong LiState Key Lab of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, 311400, China.ORCID https://orcid.org/0000-0002-1309-7732
Dawei XueCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 311121, China.ORCID https://orcid.org/0000-0001-9904-7615
Jianwei ZhangNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China.ORCID https://orcid.org/0000-0001-8030-5346
Jian ZhangState Key Lab of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, 311400, China.ORCID https://orcid.org/0000-0003-2804-0162
Yuxuan HouState Key Lab of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, 311400, China.ORCID https://orcid.org/0000-0002-0830-4717

Funding

ASTIP program of CAASBiological Breeding-National Science and Technology Major Project 2023ZD040662Major Program of Natural Science Foundation of Zhejiang LD24C130001National Key Research and Development Program of China 2024YFF1000600National Natural Science Foundation of China 32472115National Natural Science Foundation of China U22A20456National Natural Science Foundation of China W2412006Zhejiang Provincial Natural Science Foundation LY23C130004Zhejiang Provincial Outstanding Youth Science Foundation LQN25C130011
6 · The paper itself

Abstract

Touch-down overlapping PCR coupled with HiFi long-read sequencing, a high-throughput method for large-scale profiling of protein-protein interactions based on stitch-PCR identified 7,726 high-confidence interactions between rice and Arabidopsis proteins by integrating a library-vs-library yeast two-hybrid strategy with optimized PCR and long-read sequencing.

Indexed as

ArabidopsisArabidopsis ProteinsHigh-Throughput Nucleotide SequencingOryzaPolymerase Chain ReactionProtein Interaction MappingProtein Interaction MapsSpecies SpecificityTwo-Hybrid System TechniquesArabidopsis Proteins

Identifiers

PMID41345359
PMCPMC12863018

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.