Protective effects of low-dose lithium treatment on rat postural muscle under conditions of disuse.

Timur M Mirzoev, Sergey A Tyganov, Olga V Turtikova, Vitaly E Kalashnikov, Kristina A Sharlo, Boris S Shenkman

Abstract read

In one paragraph

Article in Pflugers Archiv : European journal of physiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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5 · Who and what money

Authors and funding

6 authors.

Timur M MirzoevMyology Laboratory, Institute of Biomedical Problems RAS, 123007, 76A Khoroshevskoe shosse, Moscow, Russia. tmirzoev@yandex.ru.ORCID http://orcid.org/0000-0002-1986-6221

Sergey A TyganovMyology Laboratory, Institute of Biomedical Problems RAS, 123007, 76A Khoroshevskoe shosse, Moscow, Russia.

Olga V TurtikovaMyology Laboratory, Institute of Biomedical Problems RAS, 123007, 76A Khoroshevskoe shosse, Moscow, Russia.

Vitaly E KalashnikovMyology Laboratory, Institute of Biomedical Problems RAS, 123007, 76A Khoroshevskoe shosse, Moscow, Russia.

Kristina A SharloMyology Laboratory, Institute of Biomedical Problems RAS, 123007, 76A Khoroshevskoe shosse, Moscow, Russia.

Boris S ShenkmanMyology Laboratory, Institute of Biomedical Problems RAS, 123007, 76A Khoroshevskoe shosse, Moscow, Russia.

Funding

Russian Science Foundation 17-75-20152

6 · The paper itself

Abstract

Skeletal muscles undergo significant atrophy and loss of strength under conditions of disuse/mechanical unloading. Since low-dose lithium treatment has been shown to benefit the musculoskeletal system of mammals, this study sought to investigate the effects of subtherapeutic doses of lithium chloride (LiCl) on the functional properties and markers of protein metabolism in the postural soleus muscle of rats under 7 days of mechanical unloading. Rat hindlimbs were unloaded using the hindlimb suspension (HS) method. Male Wistar rats were divided into four groups: (1) vivarium control (C), (2) vivarium control + LiCl (C + Li), (3) 7-day HS (HS), and (4) 7-day HS + LiCl (HS + Li). LiCl (40 mg/kg) was administered intraperitoneally daily throughout the experiment. Structural changes in the muscle were studied using immunohistochemistry. Markers of anabolic and catabolic processes were assessed using Western blotting and PCR. LiCl treatment during HS prevented a decrease in muscle fiber size and slow-to-fast fiber transformation. Additionally, LiCl administration during HS increased the specific maximum force and passive tension of the soleus compared to the control. LiCl administration during HS prevented decreases in GSK-3β (Ser9) and 4E-BP1 (Thr37/46) phosphorylation. Furthermore, LiCl prevented the HS-induced increase in ubiquitin, atrogin-1, MuRF-1, ULK1, and calpain-1 mRNA expression. Thus, daily low-dose LiCl administration during 7-day HS maintained the morphological parameters and increased the functional properties of the rat soleus muscle. This was accompanied by the prevention of the activation of the key proteolytic markers in the soleus muscle.

Indexed as

Lithium ChlorideMuscle, SkeletalMuscular AtrophyAnimalsGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaHindlimb SuspensionMaleMuscle ProteinsRatsRats, WistarSKP Cullin F-Box Protein LigasesGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaLithium ChlorideMuscle ProteinsSKP Cullin F-Box Protein Ligases4E-BP1DisuseGSK-3βHindlimb suspensionLiClMuscle forceProteolytic markersSoleus muscle

Identifiers

PMID41345291

DOI10.1007/s00424-025-03138-9

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.