Visualizing infection by single positive-sense RNA viruses using virus infection real-time imaging (VIRIM).

To understand viral infection and virus-host interactions, real-time, single-cell assays to track viral infection progression are essential. Many conventional assays sample large numbers of cells for single measurements, averaging out the cell-to-cell heterogeneity that is intrinsic to viral infection. Moreover, conventional assays often require cell fixation or lysis, limiting analysis to a single timepoint and masking the temporal and spatial dynamics of infection. We have developed virus infection real-time imaging (VIRIM), a method to visualize the translation of individual RNAs of viruses in real-time. The single-molecule and live-cell nature of VIRIM allows the examination of the earliest events of viral infection, when viral protein and RNA levels are still low, and allows study into the origins and consequences of cell-to-cell heterogeneity during virus infection. Here we provide a step-by-step description of the VIRIM assay, including a detailed procedure for designing, producing and validating the viruses required for VIRIM. In addition, we provide guidelines for generating the reporter cell line, performing the time-lapse imaging and analyzing the fluorescence microscopy data. Once established, a typical VIRIM experiment requires 2-5 days to complete.