Evidence map›Paper›PMID 41345140›Full record

ArticleScientific reports2025

Quantitative holographic analysis in stallion spermatozoa following cryopreservation.

Maria Antonietta Ferrara, Graziano Preziosi, Raffaele Boni, Raffaella Ruggiero, Stefano Cecchini Gualandi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Maria Antonietta FerraraInstitute of Applied Sciences and Intelligent Systems , Unit of Naples Italian National Research Council (ISASI-CNR) , Via Pietro Castellino 111, 80131, Napoli, Italy. antonella.ferrara@na.isasi.cnr.it.
Graziano PreziosiInstitute of Applied Sciences and Intelligent Systems , Unit of Naples Italian National Research Council (ISASI-CNR) , Via Pietro Castellino 111, 80131, Napoli, Italy.
Raffaele BoniDepartment of Basic and Applied Sciences (DiSBA), University of Basilicata, Via dell'Ateneo Lucano, 10, 85100, Potenza, Italy.
Raffaella RuggieroDepartment of Basic and Applied Sciences (DiSBA), University of Basilicata, Via dell'Ateneo Lucano, 10, 85100, Potenza, Italy.
Stefano Cecchini GualandiDepartment of Basic and Applied Sciences (DiSBA), University of Basilicata, Via dell'Ateneo Lucano, 10, 85100, Potenza, Italy.

Funding

Italian Ministry for University and Research (MUR) PRIN EVIDENCE - code number: 2022ERKW4P - CUP MASTER: C53D23005260006
6 · The paper itself

Abstract

This study employs Holographic tomography (HT) to examine structural and biophysical changes occurring during the cryopreservation of stallion sperm. HT is an advanced imaging technique that integrates digital holography with tomography to achieve three-dimensional, quantitative reconstructions of objects without the need for treatment or reporter dyes. By using refractive index (RI) intervals to represent specific structural regions of sperm cells, variations in optical density, surface area, volume, and dry mass across different cryopreservation extenders and donors have been quantified. Three main sperm components, (i) nuclear region, (ii) post-acrosomal region and midpiece and (iii) whole cell were identified and discriminated based on different RI. Our results revealed significant differences in volume of post-acrosomal region and midpiece among stallions as well as between fresh and frozen/thawed sperm, whereas no significant differences were observed between freezing extenders, aligning with our findings on sperm kinetics. A significant stallion-extender interaction underscores the need to personalize the sperm freezing process.

Indexed as

CryopreservationHolographySemen PreservationSpermatozoaAnimalsHorsesMaleAcrosomal integrityFreezing extenderHolographic tomographyHorseRefractive indexSperm freezing

Identifiers

PMID41345140
PMCPMC12680650

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.