Evidence map›Paper›PMID 41343670›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2025

Deciphering guanidine assimilation and riboswitch-based gene regulation in cyanobacteria for synthetic biology applications.

M Amadeus Itzenhäuser, Andreas M Enkerlin, Jan A Dewald, Bihter Avşar, Ron Stauder, Hannes Halpick, Rosalie Schaale, Lisa M Baumann, Noelia Fernandez Merayo, Thomas Maskow and 3 more

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Conversion of COMicrobial cell factories · 2026
    Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

M Amadeus ItzenhäuserDepartment of Solar Materials Biotechnology, Helmholtz Centre for Environmental Research, Leipzig 04318, Germany.ORCID 0009-0004-8553-0648
Andreas M EnkerlinMicrobial Biochemistry Group, Institute of Phototrophic Microbiology, Faculty of Mathematics and Natural Sciences, Heinrich-Heine University Düsseldorf, Düsseldorf 40225, Germany.ORCID 0000-0001-6990-820X
Jan A DewaldGeneral Biochemistry, Institute of Biochemistry and Biotechnology, Faculty of Natural Sciences 1 - Biosciences, Martin-Luther-University Halle-Wittenberg, Halle (Saale) 06120, Germany.
Bihter AvşarDepartment of Solar Materials Biotechnology, Helmholtz Centre for Environmental Research, Leipzig 04318, Germany.ORCID 0000-0001-6029-2330
Ron StauderDepartment of Solar Materials Biotechnology, Helmholtz Centre for Environmental Research, Leipzig 04318, Germany.ORCID 0000-0002-8237-2670
Hannes HalpickDepartment of Solar Materials Biotechnology, Helmholtz Centre for Environmental Research, Leipzig 04318, Germany.
Rosalie SchaaleDepartment of Solar Materials Biotechnology, Helmholtz Centre for Environmental Research, Leipzig 04318, Germany.
Lisa M BaumannDepartment of Solar Materials Biotechnology, Helmholtz Centre for Environmental Research, Leipzig 04318, Germany.ORCID 0009-0001-3092-5018
Noelia Fernandez MerayoDepartment of Microbial Biotechnology, Helmholtz Centre for Environmental Research, Leipzig 04318, Germany.
Thomas MaskowDepartment of Microbial Biotechnology, Helmholtz Centre for Environmental Research, Leipzig 04318, Germany.
Khaled A SelimMicrobial Biochemistry Group, Institute of Phototrophic Microbiology, Faculty of Mathematics and Natural Sciences, Heinrich-Heine University Düsseldorf, Düsseldorf 40225, Germany.ORCID 0000-0002-2974-9483
Christina E WeinbergGeneral Biochemistry, Institute of Biochemistry and Biotechnology, Faculty of Natural Sciences 1 - Biosciences, Martin-Luther-University Halle-Wittenberg, Halle (Saale) 06120, Germany.
Stephan KlähnDepartment of Solar Materials Biotechnology, Helmholtz Centre for Environmental Research, Leipzig 04318, Germany.ORCID 0000-0002-2933-486X

Funding

Deutsche Forschungsgemeinschaft (DFG) KL 3114/10-1Deutsche Forschungsgemeinschaft (DFG) KL 3114/7-1Deutsche Forschungsgemeinschaft (DFG) SE 3449/1-1Deutsche Forschungsgemeinschaft (DFG) SE 3449/3-1
6 · The paper itself

Abstract

Guanidine is well known as a denaturing agent. However, recent studies have demonstrated both the widespread synthesis of guanidine, e.g., in plants and mammals, as well as the widespread occurrence of guanidine metabolism in bacteria, suggesting a broader biological role. Here, we provide insights into guanidine assimilation via guanidine hydrolases (GdmH) in cyanobacteria. The

Indexed as

CyanobacteriaGene Expression Regulation, BacterialGuanidineRiboswitchSynthetic BiologyATP-Binding Cassette TransportersBacterial ProteinsNitrogenPromoter Regions, GeneticATP-Binding Cassette TransportersBacterial ProteinsGuanidineNitrogenRiboswitchABC transportgene regulationguanidine riboswitchmolecular engineeringnitrogen assimilation

Identifiers

PMID41343670
PMCPMC12704784

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.