ArticlePLoS genetics2025
Single-cell transcriptomics reveals stepwise transformation of epithelial cells into Non-Professional Phagocytes.
Article in PLoS genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Danger at the border: Rac turns nibblers into murderers.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
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Authors and funding
4 authors.
Funding
Abstract
The differentiation of epithelial cells into Non-Professional Phagocytes (NPPs) is essential for maintaining tissue homeostasis and clearing apoptotic debris. In the Drosophila ovary, epithelial follicle cells transform into NPPs following germline cell death, but the genetic mechanisms controlling this transition are not well defined. To investigate these mechanisms, we used a model in which overexpression of the active form of Notch, the Notch Intracellular Domain (NICD), induces a robust epithelial-to-NPP transition. Using single-cell RNA sequencing and trajectory analysis, we identified three transcriptional phases of NPP maturation: an early stage of metabolic activation, an intermediate stage enriched in genes related to migration and cytoskeletal remodeling, and a late stage marked by autophagy-related gene expression. These transcriptomic patterns were validated by immunostaining. SCENIC and ChiP-seq analyses identified the JNK effector Jun-related antigen (Jra) and its predicted targets, Arp2 and Arp3, which encode components of the Arp2/3 complex, as regulators of cytoskeletal remodeling. Functional assays confirmed that the JNK-Jra-Arp2/3 axis is required for cytoplasmic expansion and debris clearance during NPP differentiation.
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