Evidence map›Paper›PMID 41342880›Full record

ArticleThe Journal of experimental medicine2026

Notch interaction with RUNX factors regulates initiation of the T-lineage program.

Yuichi Kama, Ken-Ichi Hirano, Kaori Masuhara, Yusuke Endo, Yuka Suzuki, Masanori Fujimoto, Tatsuma Matsuda, Takashi Yahata, Masahiko Kato, Katsuto Hozumi and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of experimental medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yuichi KamaDepartment of Immunology, Tokai University School of Medicine, Isehara, Japan.ORCID 0000-0002-9294-9429
Ken-Ichi HiranoDepartment of Immunology, Tokai University School of Medicine, Isehara, Japan.ORCID 0000-0003-3495-610X
Kaori MasuharaDepartment of Immunology, Tokai University School of Medicine, Isehara, Japan.ORCID 0000-0002-6648-2431
Yusuke EndoLaboratory of Medical Omics Research, Kazusa DNA Research Institute, Kisarazu, Japan.ORCID 0000-0002-7223-1938
Yuka SuzukiDepartment of Immunology, Tokai University School of Medicine, Isehara, Japan.ORCID 0009-0002-8541-3949
Masanori FujimotoDepartment of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chuo-ku, Japan.ORCID 0000-0003-1458-8207
Tatsuma MatsudaDepartment of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chuo-ku, Japan.ORCID 0000-0002-9451-1092
Takashi YahataTranslational Molecular Therapeutics Laboratory, Division of Host Defense Mechanism, Tokai University School of Medicine, Isehara, Japan.ORCID 0000-0002-9332-0358
Masahiko KatoTokai University School of Medicine , Isehara, Japan.ORCID 0000-0002-8815-8552
Katsuto HozumiDepartment of Immunology, Tokai University School of Medicine, Isehara, Japan.ORCID 0000-0002-7685-6927
Tomoaki TanakaDepartment of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chuo-ku, Japan.ORCID 0000-0002-9761-1750
Hiroyuki HosokawaDepartment of Immunology, Tokai University School of Medicine, Isehara, Japan.ORCID 0000-0002-9592-2889

Funding

Chemo-Sero-Therapeutic Research InstituteChugai Foundation for Innovative Drug Discovery ScienceFoundation for Promotion of Cancer ResearchIchiro Kanehara Foundation for the Promotion of Medical Science and Medical CareJapan Society for the Promotion of Science JP19H03692Japan Society for the Promotion of Science JP23K15307Japan Society for the Promotion of Science JP24K02485Japan Society for the Promotion of Science JP24K22062Kawano Masanori Memorial Public Interest Incorporated Foundation for Promotion of Pediatrics 35-y10Kobayashi FoundationKyushu UniversityMitsubishi FoundationNaito FoundationPrincess Takamatsu Cancer Research FundSENSHIN Medical Research FoundationTakeda Science FoundationTERUMO Life Science FoundationTokai UniversityTokai University School of MedicineUehara Memorial FoundationVehicle Racing Commemorative Foundation
6 · The paper itself

Abstract

Runt-related transcription (RUNX) factors play a key role in T cell development. At the T-lineage commitment checkpoint, RUNX1 undergoes dynamic partner switching, resulting in its redeployment. Here, we investigated the functional differences in RUNX factors between the lymphoid progenitor (LP)- and Notch-stimulated earliest T progenitor stages (Phase 1). We identified CCCTC-binding factor (CTCF) as an LP-specific RUNX1-interacting partner, with LP-specific RUNX1-binding genomic sites significantly enriched for CTCF consensus motifs and co-occupied by CTCF. On Notch stimulation, Notch1 intracellular domain directly interacts with RUNX1 and recruits the RUNX1/Mediator/p300 transcriptional activation complex to Notch-regulated T-signature gene loci. CRISPR/Cas9-mediated stage-specific deletion of RUNX factors and their binding partners revealed that the RUNX1/CTCF complex in LP negatively regulates T-signature gene expression, whereas the RUNX1/Mediator/p300 complex in Phase 1 promotes it. Our findings highlight the crucial role of Notch-mediated functional conversion of RUNX factors, including protein complex reorganization and genomic redeployment in initiating T-lineage program.

Indexed as

CCCTC-Binding FactorCell LineageCore Binding Factor Alpha 2 SubunitReceptor, Notch1T-LymphocytesAnimalsCRISPR-Cas SystemsE1A-Associated p300 ProteinFemaleGene Knock-In TechniquesLymphoid Progenitor CellsMaleMediator ComplexMiceMice, TransgenicProtein BindingCCCTC-Binding FactorCore Binding Factor Alpha 2 SubunitCtcf protein, mouseE1A-Associated p300 ProteinEbf1 protein, mouseEp300 protein, mouseMediator ComplexNotch1 protein, mouseReceptor, Notch1Runx1 protein, mouseTrans-Activators

Identifiers

PMID41342880
PMCPMC12677141

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.