Preclinical validation of magnetic hyperthermia therapy for locally advanced oral cancer treatment.

Locally advanced tumors of the buccal cavity harbor abundant hypoxic and cancer stem cell populations, rendering them intrinsically resistant to conventional radiotherapy and chemotherapy. Current aggressive treatment regimens, though effective in part, often result in collateral tissue injury, functional impairment, and significant morbidity. Magnetic hyperthermia (MHT)-a minimally invasive technique that employs magnetic nanoparticles (MNPs) to generate localized heat under an alternating magnetic field (AMF)-represents a promising alternative, as its therapeutic efficacy is independent of tumor cell type or microenvironmental conditions. Despite extensive preclinical progress, clinical translation has been hindered by safety and feasibility challenges, particularly the reliance on non-translatable, high-frequency AMF systems. To address these hurdles, this study evaluated magnetic hyperthermia for oral squamous cell carcinoma (OSCC) using preclinical models and a scaled-up AMF generator designed within human safety thresholds, unlike prior studies that used non-translatable high-frequency fields. FDA-approved amino silane-coated Fe