Effects of added exogenous hormones on lactation-related physiological functions of equine mammary epithelial cells.

Chao Li, Shengchen Zheng, Jianwei Lin, Qian Li, Kailun Yang, Xiaobin Li

Abstract read

In one paragraph

Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Chao Li *Xinjiang Herbivore Nutrition Laboratory for Meat & Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi, China.

Shengchen Zheng *Xinjiang Herbivore Nutrition Laboratory for Meat & Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi, China.

Jianwei LinXinjiang Herbivore Nutrition Laboratory for Meat & Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi, China.

Qian LiXinjiang Herbivore Nutrition Laboratory for Meat & Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi, China.

Kailun YangXinjiang Herbivore Nutrition Laboratory for Meat & Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi, China.

Xiaobin LiXinjiang Herbivore Nutrition Laboratory for Meat & Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to optimize the culture conditions for equine mammary epithelial cells (EMECs) by investigating the effects of fetal bovine serum (FBS), hydrocortisone (HYD), insulin (INS), and epidermal growth factor (EGF) on cell growth and function. The primary objectives were to identify the optimal culture conditions for EMECs, evaluate the impact of FBS, HYD, INS, and EGF on cell viability and milk component synthesis, and uncover genes involved in mare lactation and milk production. The optimal FBS concentration for cell survival was determined to be 15%, with further improvements achieved through the individual addition of 1 μg/mL HYD, 5 μg/mL INS, and 5 ng/mL EGF. Orthogonal analysis revealed that the combination of 1 μg/mL HYD and 5 ng/mL EGF resulted in the highest survival rates. This combination significantly increased triglyceride and lactose production by 86.36% and 33.33%, respectively (

Indexed as

cell viabilityepidermal growth factorequine mammary epithelial cellshydrocortisonetranscriptome

Identifiers

PMID41340932

DOI10.3389/fvets.2025.1660502

PMCPMC12671389

What OpenQuestion holds

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LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.