Evidence map›Paper›PMID 41340924›Full record

ArticleRegenerative therapy2026

Butyrate-preconditioned human adipose-derived stem cell-conditioned medium enhances myocardial perfusion after infarction.

Shinji Kobuchi, Wan-Tseng Hsu, Misaki Matsuzawa, Rina Kagawa, Junko Watanabe, Koki Harada, Yuki Toda, Shohei Hamada, Masayuki Tsujimoto, Hidekazu Kawashima and 3 more

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Article in Regenerative therapy, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Shinji KobuchiLaboratory of Pharmacokinetics, Kyoto Pharmaceutical University, Kyoto, Japan.
Wan-Tseng HsuSchool of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan.
Misaki MatsuzawaJoint Research Laboratory, Division of Integrated Pharmaceutical Sciences, Kyoto Pharmaceutical University, Kyoto, Japan.
Rina KagawaJoint Research Laboratory, Division of Integrated Pharmaceutical Sciences, Kyoto Pharmaceutical University, Kyoto, Japan.
Junko WatanabeJoint Research Laboratory, Division of Integrated Pharmaceutical Sciences, Kyoto Pharmaceutical University, Kyoto, Japan.
Koki HaradaJoint Research Laboratory, Division of Integrated Pharmaceutical Sciences, Kyoto Pharmaceutical University, Kyoto, Japan.
Yuki TodaLaboratory of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Kyoto, Japan.
Shohei HamadaLaboratory of Pharmaceutical Chemistry, Kyoto Pharmaceutical University, Kyoto, Japan.
Masayuki TsujimotoLaboratory of Clinical Pharmacy, Kyoto Pharmaceutical University, Kyoto, Japan.
Hidekazu KawashimaRadioisotope Research Center, Division of Integrated Pharmaceutical Sciences, Kyoto Pharmaceutical University, Kyoto, Japan.
Kaneyasu NishimuraLaboratory of Functional Brain Circuit Construction, Graduate School of Brain Science, Doshisha University, Kyotanabe, Japan.
Kenjiro MatsumotoLaboratory of Pathophysiology, Faculty of Pharmaceutical Sciences, Doshisha Women's College of Liberal Arts, Kodo Kyotanabe, Kyoto, Japan.
Kazuyuki TakataJoint Research Laboratory, Division of Integrated Pharmaceutical Sciences, Kyoto Pharmaceutical University, Kyoto, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Adipose-derived stem cell-conditioned medium (ASC-CM) is promising for cardiac repair via paracrine mechanisms. However, variability in efficacy limits its clinical translation. We investigated whether preconditioning human ASC with butyrate (ASC-BA-CM) enhanced its paracrine potency and improved Method: RNA-sequencing of human ASCs treated with butyrate was performed to characterize transcriptomic changes. CM was collected and analyzed via cytokine/chemokine arrays. Wound healing assays using human umbilical vein endothelial cells (HUVECs), with and without THP-1 macrophage co-culture, were performed to evaluate endothelial repair and its correlations with secreted factors. Results: Butyrate preconditioning upregulated angiogenesis- and immune-related genes, including Conclusions: Butyrate preconditioning enhanced the paracrine activity of ASC-CM and was associated with improved myocardial perfusion in a rat model. These findings suggest that butyrate may augment the ASC secretome function. Potential mechanisms such as endothelial repair, angiogenesis, and immune modulation remain hypothetical and require further validation in future studies.

Indexed as

Adipose-derived stem cellsAngiogenesisButyrateMyocardial infarctionSecretomeSPECT/CT

Identifiers

PMID41340924
PMCPMC12670594

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.