Evidence map›Paper›PMID 41339680›Full record

ArticleScientific reports2025

High-throughput isolation of normal and pulmonary fibrosis human alveolar epithelial cells.

Anthony L Eiliazadeh, Melissa A V Suma, Jennifer Li, Marcelo Cuesta, Junaid Ishaq, Daisuke Taniguchi, Shaf Keshavjee, Thomas K Waddell, Golnaz Karoubi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Hallmarks of the ageing lung: 10 years later.The European respiratory journal · 2026
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Anthony L EiliazadehLatner Thoracic Research Laboratories, Toronto General Hospital Research Institute, University Health Network, 101 College Street, Toronto, ON, M5G1L7, Canada.
Melissa A V SumaLatner Thoracic Research Laboratories, Toronto General Hospital Research Institute, University Health Network, 101 College Street, Toronto, ON, M5G1L7, Canada.
Jennifer LiLatner Thoracic Research Laboratories, Toronto General Hospital Research Institute, University Health Network, 101 College Street, Toronto, ON, M5G1L7, Canada.
Marcelo CuestaLatner Thoracic Research Laboratories, Toronto General Hospital Research Institute, University Health Network, 101 College Street, Toronto, ON, M5G1L7, Canada.
Junaid IshaqMichael G. DeGroote School of Medicine, McMaster University, 90 Main Street West, Hamilton, ON, L8P 1H6, Canada.
Daisuke TaniguchiDepartment of Surgical Oncology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
Shaf KeshavjeeLatner Thoracic Research Laboratories, Toronto General Hospital Research Institute, University Health Network, 101 College Street, Toronto, ON, M5G1L7, Canada.
Thomas K WaddellLatner Thoracic Research Laboratories, Toronto General Hospital Research Institute, University Health Network, 101 College Street, Toronto, ON, M5G1L7, Canada.
Golnaz KaroubiLatner Thoracic Research Laboratories, Toronto General Hospital Research Institute, University Health Network, 101 College Street, Toronto, ON, M5G1L7, Canada. golnaz.karoubi@utoronto.ca.

Funding

CIHR 407666
6 · The paper itself

Abstract

Human alveolar type 2 (AT2) cells play a crucial role in maintaining lung homeostasis and facilitating repair. Researchers are investigating AT2 cells in ex vivo platforms due to their essential roles in lung physiology, disease modeling, and drug testing. Herein, we present a comprehensive workflow for the isolation and culture of primary human AT2 cells, including an efficient enzyme-based lung tissue digestion protocol and automated purification of AT2 cells from both healthy and diseased lungs. By targeting the HTII-280 surface protein on AT2 cells, we employed an automated magnetic-activated cell sorting (MACS) separation strategy using the autoMACS® Pro Separator. AT2 cells from healthy and diseased lungs were grown in 3D culture and formed alveolospheres, elucidating that the isolation strategy did not affect cell functionality. This approach provides high AT2 cell yield and purity, offering scalability to generate sufficient quantities of cells for downstream applications, such as disease modeling and cell-based therapeutics.

Indexed as

Alveolar Epithelial CellsCell SeparationPulmonary FibrosisCells, CulturedHumansLungAlveolar type 2 cellsCell isolation efficiencyLung cell isolationMagnetic-activated cell sorting

Identifiers

PMID41339680
PMCPMC12780255

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.