Evidence map›Paper›PMID 41338122›Full record

ArticleInternational dental journal2026

LOX From Salivary Adenoid Cystic Carcinoma-Associated Fibroblast Promotes Fibrosis in the Pulmonary Pre-metastatic Niche.

Kabulo Katshi Cedric, Shengqiao Sun, Dong Jin, Song Bao, Bo Sun, Jinwen Zhou, Tingjiao Liu, Jing Kong

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PubMed Full text at PMC Publisher
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Article in International dental journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Kabulo Katshi CedricSchool of Stomatology, Dalian Medical University, Dalian, China.
Shengqiao SunThe Fourth Department of Circulation, Affiliated Zhongshan Hospital of Dalian University, Dalian, China.
Dong JinSchool of Stomatology, Dalian Medical University, Dalian, China.
Song BaoSchool of Stomatology, Dalian Medical University, Dalian, China; The Affiliated Stomatological Hospital of Dalian Medical University, Dalian, China.
Bo SunDepartment of Oral Surgery, the Second Affiliated Hospital of Dalian Medical University, Dalian, China.
Jinwen ZhouSchool of Stomatology, Dalian Medical University, Dalian, China; The Affiliated Stomatological Hospital of Dalian Medical University, Dalian, China.
Tingjiao LiuDepartment of Oral Pathology, Shanghai Stomatological Hospital & School of Stomatology, Fudan University, Shanghai, China. Electronic address: tingjiao_liu@fudan.edu.cn.
Jing KongSchool of Stomatology, Dalian Medical University, Dalian, China. Electronic address: jingk@dmu.edu.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimsMetastasis is the primary cause of cancer-related mortality. Carcinoma-associated fibroblasts (CAFs) critically promote lung metastasis in salivary adenoid cystic carcinoma (SACC). CAFs within metastatic organs, often termed metastasis-associated fibroblasts (MAFs), drive extracellular matrix (ECM) remodelling during metastasis. Understanding early ECM remodelling events in the metastatic niche may offer crucial insights for inhibiting metastasis. However, the specific mechanisms through which SACC-associated CAFs regulate lung ECM remodelling remain poorly understood.

methodsCAFs and collagen deposition in murine tissues were visualised using H&E and picrosirius red staining. The role of lysyl oxidase (LOX) in CAF-mediated collagen cross-linking and its underlying molecular mechanisms were examined through western blotting, immunofluorescence staining and collagen contraction assays.

resultsIn this study, we showed that CAFs enhanced collagen cross-linking, leading to stromal fibrosis during the establishment of the pre-metastatic niche and subsequent SACC lung metastasis. LOX, a copper-dependent enzyme crucial for collagen cross-linking and secreted by CAFs, promotes the activation of lung MAFs and increases their collagen I expression. MAF activation is facilitated by LOX, which induces YAP expression and its nuclear translocation. β-Aminopropionitrile, a specific LOX inhibitor, effectively suppressed fibroblast activation and reduced nuclear YAP expression.

conclusionsOur findings indicate that soluble LOX secreted by CAFs promotes metastatic progression through the induction of pulmonary fibrosis. Targeting this LOX-mediated pathway may represent a promising therapeutic strategy for preventing lung metastasis in patients with SACC. Furthermore, given the complexity of metastasis, future studies should investigate how fibrosis influences key stages of metastatic colony formation, including the extravasation and dormancy of circulating tumour cells.

Indexed as

Cancer-Associated FibroblastsCarcinoma, Adenoid CysticLung NeoplasmsProtein-Lysine 6-OxidaseSalivary Gland NeoplasmsAnimalsCollagenExtracellular MatrixFibroblastsFibrosisHumansMiceCollagenProtein-Lysine 6-OxidaseCarcinoma-associated fibroblastsCollagen cross-linkingLysyl oxidasePre-metastatic nicheSalivary adenoid cystic carcinomaYes-associated protein

Identifiers

PMID41338122
PMCPMC12718463

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.