ArticleMycology2025
Application of a One-Step method for rapid detection of nucleic acids from fungi.
Article in Mycology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Clinical diagnostic value of targeted next generation sequencing for lower respiratory tract infection: a retrospective study.Frontiers in cellular and infection microbiology · 2026Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PCR-based techniques play a crucial role in genotyping and genetic screening in fungal biology. Rapid access to nucleic acids for these reactions can significantly improve the efficiency of fungal analysis, especially when multiple samples need to be tested. In this study, we introduced a simple and rapid method for detecting small amounts of fungal DNA or RNA, named the One-Step method, and confirmed its applicability across various experimental scenarios for fungal detection. The method involves scraping a small quantity of spores or mycelium into sterile water, followed by heat shock, vortexing, and centrifugation to obtain a supernatant that serves as a template for the PCR reaction. Notably, nucleic acids were successfully extracted using the One-Step method from four different genera of fungi-
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Registered trials
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