Evidence map›Paper›PMID 41333722›Full record

ArticleVeterinary world2025

Comparative immunodiagnostic evaluation of recombinant serine proteases from

Aibek Zhumalin, Aisarat Gajimuradova, Aitbay Bulashev, Orken Akibekov, Alfiya Syzdykova, Nurtai Gubaidullin, Fabio Tosini, Fariza Zhagipar, Ali Aydin, Nasipkhan Askarova

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary world, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Aibek ZhumalinDepartment of Veterinary Medicine, Faculty of Veterinary and Livestock Technology, S. Seifullin Kazakh Agrotechnical Research University, 62 Zhenis Avenue, Astana, 010011, Kazakhstan.
Aisarat GajimuradovaOrganic Agrotechnology Engineering Center, Agrotechnical park Seifullin University, S. Seifullin Kazakh Agrotechnical Research University, 62 Zhenis Avenue, Astana, 010011, Kazakhstan.
Aitbay BulashevDepartment of Microbiology and Biotechnology, Faculty of Veterinary and Livestock Technology, S. Seifullin Kazakh Agrotechnical Research University, 62 Zhenis Avenue, Astana, 010011, Kazakhstan.
Orken AkibekovDepartment of Microbiology and Biotechnology, Faculty of Veterinary and Livestock Technology, S. Seifullin Kazakh Agrotechnical Research University, 62 Zhenis Avenue, Astana, 010011, Kazakhstan.
Alfiya SyzdykovaOrganic Agrotechnology Engineering Center, Agrotechnical park Seifullin University, S. Seifullin Kazakh Agrotechnical Research University, 62 Zhenis Avenue, Astana, 010011, Kazakhstan.
Nurtai GubaidullinOrganic Agrotechnology Engineering Center, Agrotechnical park Seifullin University, S. Seifullin Kazakh Agrotechnical Research University, 62 Zhenis Avenue, Astana, 010011, Kazakhstan.
Fabio TosiniEuropean Union Reference Laboratory for Parasites, "lstituto Superiore di Sanità", 299, Viale Regina Elena, Roma, 00161, Italy.
Fariza ZhagiparOrganic Agrotechnology Engineering Center, Agrotechnical park Seifullin University, S. Seifullin Kazakh Agrotechnical Research University, 62 Zhenis Avenue, Astana, 010011, Kazakhstan.
Ali AydinDivision of Food Hygiene and Technology, Faculty of Veterinary Medicine, İstanbul University-Cerrahpasa, Üniversite MAhallesi, Üniversite Caddesi, 34320, Avcılar, İstanbul, Turkiye.
Nasipkhan AskarovaOrganic Agrotechnology Engineering Center, Agrotechnical park Seifullin University, S. Seifullin Kazakh Agrotechnical Research University, 62 Zhenis Avenue, Astana, 010011, Kazakhstan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background and Aim: Trichinellosis, a major zoonotic foodborne disease caused by Materials and Methods: Serine protease genes of Results: Both recombinant proteins were successfully expressed at expected sizes (10.8 kDa for rTsp-LE; 6.8 kDa for rTnsp-4E) and induced strong, specific antibody responses. Antibodies against rTsp-LE and rTnsp-4E were detectable as early as 7 dpi, earlier than ES antigens (14-21 dpi). Immunized mice showed significant titers (up to 1:5700 for rTsp-LE and 1:10400 for rTnsp-4E by day 30). No cross-reactivity was observed with Conclusion: Recombinant serine proteases rTsp-LE and rTnsp-4E demonstrated high sensitivity, species-specific reactivity, and early antibody detection, outperforming ES antigens. These findings support their potential as reliable candidates for serological assays, contributing to earlier and more accurate trichinellosis diagnosis and improved epidemiological surveillance.

Indexed as

immunodiagnosisrecombinant antigensserine proteaseTrichinella nativaTrichinella spiralistrichinellosis

Identifiers

PMID41333722
PMCPMC12668753

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.