ArticleBiochemistry and biophysics reports2025
Analyzing qPCR data: Better practices to facilitate rigor and reproducibility.
Article in Biochemistry and biophysics reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed.
- Inflammatory biomarker alterations and Caspase-3 gene expression in patients with Entamoeba spp. infection.Molecular biology reports · 2026Article
- Matrix- and Differentiation Stage-Dependent Variability of Reference Genes: Rethinking Validation Strategies in 3T3-L1 Adipogenic Models.International journal of molecular sciences · 2026Article
- Effects ofExperimental and therapeutic medicine · 2026Article
- Advances in algorithms for normalizer gene selection in qRT-PCR: implications for cancer biology and precision medicine.Frontiers in genetics · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative PCR (qPCR) remains a widely used, cost-effective method for RNA quantitation, yet many published studies inadequately comply with MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) and FAIR (Findable, Accessible, Interoperable, Reproducible) data principles. Widespread reliance on the 2
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.