Evidence map›Paper›PMID 41332620›Full record

ArticlebioRxiv : the preprint server for biology2025

Orchestrating Spatial Transcriptomics Analysis with Bioconductor.

Helena L Crowell, Yixing Dong, Ilaria Billato, Peiying Cai, Martin Emons, Samuel Gunz, Boyi Guo, Mengbo Li, Alexandru Mahmoud, Artür Manukyan and 24 more

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

34 authors.

Helena L CrowellNational Center for Genomic Analysis, Barcelona, Spain.ORCID 0000-0002-4801-1767
Yixing DongBiomedical Data Science Center, Lausanne University Hospital, Lausanne, Switzerland.ORCID 0009-0003-5115-5686
Ilaria BillatoDepartment of Biology, University of Padova, Padova, Italy.ORCID 0000-0002-3335-3254
Peiying CaiDepartment of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.ORCID 0009-0001-9229-2244
Martin EmonsDepartment of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.ORCID 0009-0000-5219-5311
Samuel GunzDepartment of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.ORCID 0000-0002-8909-0932
Boyi GuoDivision of Biostatistics, Department of Population Health Sciences, University of Utah, Salt Lake City, UT, United States.ORCID 0000-0003-2950-2349
Mengbo LiBioinformatics and Computational Biology Division, Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.ORCID 0000-0002-9666-5810
Alexandru MahmoudChanning Division of Network Medicine, Mass General Brigham, Boston, MA, United States.ORCID 0000-0002-3779-492X
Artür ManukyanBerlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany.ORCID 0000-0002-0441-9517
Hervé PagèsFred Hutch Cancer Center, Seattle, WA, United States.ORCID 0009-0002-8272-4522
Pratibha PanwarSchool of Mathematics and Statistics, The University of Sydney, Camperdown, NSW, Australia.ORCID 0000-0002-7437-7084
Shreya RaoSchool of Mathematics and Statistics, The University of Sydney, Camperdown, NSW, Australia.ORCID 0009-0007-8140-6844
Callum J SargeantBioinformatics and Computational Biology Division, Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.ORCID 0009-0006-8919-608X
Lori Shepherd KernRoswell Park Comprehensive Cancer Center, Buffalo, NY, United States.ORCID 0000-0002-5910-4010
Marcel RamosInstitute for Implementation Science in Population Health, City University of New York Graduate School of Public Health and Health Policy, New York, NY, United States.ORCID 0000-0002-3242-0582
Jieran SunBiomedical Data Science Center, Lausanne University Hospital, Lausanne, Switzerland.ORCID 0000-0002-7996-3840
Michael TottyDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States.ORCID 0000-0002-9292-8556
Vincent J CareyChanning Division of Network Medicine, Mass General Brigham, Boston, MA, United States.ORCID 0000-0003-4046-0063
Yunshun ChenBioinformatics and Computational Biology Division, Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.ORCID 0000-0003-4911-5653
Leonardo Collado-TorresDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States.ORCID 0000-0003-2140-308X
Shila GhazanfarSchool of Mathematics and Statistics, The University of Sydney, Camperdown, NSW, Australia.ORCID 0000-0001-7861-6997
Kasper D HansenDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States.ORCID 0000-0003-0086-0687
Keri MartinowichLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, United States.ORCID 0000-0002-5237-0789
Kristen R MaynardLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, United States.ORCID 0000-0003-0031-8468
Ellis PatrickSchool of Mathematics and Statistics, The University of Sydney, Camperdown, NSW, Australia.ORCID 0000-0002-5253-4747
Dario RighelliDepartment of Electrical Engineer and Information Technology, University of Naples "Federico II", Naples, Italy.ORCID 0000-0003-1504-3583
Davide RissoDepartment of Statistical Sciences, University of Padova, Padova, Italy.ORCID 0000-0001-8508-5012
Simone TiberiDepartment of Statistical Sciences, University of Bologna, Bologna, Italy.ORCID 0000-0002-3054-9964
Levi WaldronInstitute for Implementation Science in Population Health, City University of New York Graduate School of Public Health and Health Policy, New York, NY, United States.ORCID 0000-0003-2725-0694
Raphael GottardoBiomedical Data Science Center, Lausanne University Hospital, Lausanne, Switzerland.ORCID 0000-0002-3867-0232
Mark D RobinsonDepartment of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.ORCID 0000-0002-3048-5518
Stephanie C HicksDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States.ORCID 0000-0002-7858-0231
Lukas M WeberDepartment of Biostatistics, Boston University School of Public Health, Boston, MA, United States.ORCID 0000-0002-3282-1730

Funding

Supplement: Enhancing Community Contributions to Bioconductor With Build System Containerization and a GPU for TestingU24HG004059 · NHGRI · DANA-FARBER CANCER INST · PI VINCENT JAMES CAREY, Rafael Angel Irizarry · 2021 to 2026
$7.3M
Laminar dissection of cortical human brain gene expression in neuropsychiatric disordersR01MH126393 · NIMH · LIEBER INSTITUTE, INC. · PI MARTINOWICH, KERI · 2021 to 2025
$3.9M
Spatial registration of gene expression in the human brainU01MH122849 · NIMH · LIEBER INSTITUTE, INC. · PI MARTINOWICH, KERI · 2020 to 2021
$1.7M
Computational Methods for Emerging Spatially-resolved Transcriptomics with Multiple SamplesR35GM150671 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Stephanie Carinne Hicks · 2023 to 2026
$1.2M
Unsupervised Statistical Methods for Data-driven Analyses in Spatially Resolved Transcriptomics DataR00HG012229 · NHGRI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI Lukas Martin Weber · 2024 to 2026
$730k
NHGRI NIH HHS R00 HG012229NHGRI NIH HHS U24 HG004059NIGMS NIH HHS R35 GM150671NIMH NIH HHS R01 MH126393NIMH NIH HHS U01 MH122849
6 · The paper itself

Abstract

Spatial transcriptomics technologies provide spatially-resolved measurements of gene expression through assays that can either target selected genes or capture transcriptome-wide expression profiles. The complexity and variability of these technologies and their associated data necessitate multi-step workflows integrating diverse computational methods and software packages. We provide a freely accessible, open-source, continuously updated and tested online book containing reproducible code examples, datasets, and discussion about data analysis workflows for spatial omics data using Bioconductor in R, including interoperability with Python.

Indexed as

Bioconductorcomputational biologydata analysisgene expressionhigh-dimensional datainteroperabilityopen-sourceRreproducible researchspatially-resolved transcriptomicsspatial omicsspatial transcriptomicsworkflow

Identifiers

PMID41332620
PMCPMC12667768

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.