Evidence map›Paper›PMID 41332595›Full record

ArticlebioRxiv : the preprint server for biology2025

Engineering ATP Import in Yeast Uncovers a Synthetic Route to Extend Cellular Lifespan.

Naci Oz, Hetian Su, Vedat Sari, Praveen Patnaik, Rohil Hameed, Jong Hee Song, Derek C Prosser, Vyacheslav M Labunskyy, Vadim N Gladyshev, Nan Hao and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Naci OzSchool of Life Sciences and Sustainability, Virginia Commonwealth University, Richmond, VA, USA.
Hetian SuDepartment of Molecular Biology, University of California San Diego, La Jolla, CA, USA.
Vedat SariDepartment of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA, USA.
Praveen PatnaikDepartment of Dermatology, Boston University School of Medicine, Boston, USA.
Rohil HameedDepartment of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA, USA.
Jong Hee SongBiomolecular Analysis Facility, School of Medicine, University of Virginia, Charlottesville, VA 22903, USA.
Derek C ProsserSchool of Life Sciences and Sustainability, Virginia Commonwealth University, Richmond, VA, USA.
Vyacheslav M LabunskyyDepartment of Dermatology, Boston University School of Medicine, Boston, USA.
Vadim N GladyshevDepartment of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Nan HaoDepartment of Molecular Biology, University of California San Diego, La Jolla, CA, USA.
Alaattin KayaSchool of Life Sciences and Sustainability, Virginia Commonwealth University, Richmond, VA, USA.

Funding

Molecular mechanisms of translational regulation in agingR01AG058713 · NIA · BOSTON UNIVERSITY MEDICAL CAMPUS · PI LABUNSKYY, VYACHESLAV M · 2019 to 2023
$2.9M
Reprogramming cell-fate decisions through predictive modeling and synthetic biologyR01GM144595 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HAO, NAN, HASTY, JEFF M · 2022 to 2025
$2.8M
Engineered genetic clocks for control of cellular agingR01AG086348 · NIA · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI JEFF M HASTY, Nan Hao · 2024 to 2026
$2.0M
The role of mito-nuclear communication in the adaptation to mitochondrial dysfunction and stress resistanceR35GM150858 · NIGMS · VIRGINIA COMMONWEALTH UNIVERSITY · PI Alaattin Kaya · 2023 to 2026
$1.8M
Understanding the Causes of AgingR01AG038004 · NIA · BRIGHAM AND WOMEN'S HOSPITAL · PI GLADYSHEV, VADIM N. · 2010 to 2013
$1.1M
NIA NIH HHS R01 AG038004NIA NIH HHS R01 AG058713NIA NIH HHS R01 AG086348NIGMS NIH HHS R01 GM144595NIGMS NIH HHS R35 GM150858
6 · The paper itself

Abstract

Aging results from the gradual accumulation of molecular damage as a result of cellular processes and is characterized by impaired functions, most notably an age-related decline in ATP production. However, the causal relationship between cellular ATP homeostasis and aging has not been established. In this study, we used a novel approach by harnessing a nucleotide transporter from a eukaryotic intracellular parasite to facilitate the direct import of extracellular ATP into budding yeast cells, enabling us to effectively manipulate their intracellular ATP levels. We found that depletion of ATP significantly reduces lifespan, while the supplementation of ATP in the growth medium fully restores it thereby extending lifespan. Moreover, gene expression analysis revealed that elevated ATP levels inhibit catabolic processes, indicating a suppression of glucose metabolism. Overall, our study revealed the direct impact of cellular ATP homeostasis on lifespan regulation that has never been directly tested before. This work offers new insights into the bioenergetic control of aging and positions energy metabolism as a promising target for longevity interventions.

Indexed as

AgingATPEnergy metabolismMitochondriaNucleotide transportSaccharomyces cerevisiae

Identifiers

PMID41332595
PMCPMC12667902

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.