Evidence map›Paper›PMID 41332539›Full record

ArticlebioRxiv : the preprint server for biology2025

Spatial regulation of AMPK activity under oxidative stress requires LKB1.

Kasey Parks, Arnav Jhawar, Alexia Andrikopoulos, Declan M Winters, Teagan S Dean, Edmund D Kapelczak, Tara TeSlaa, Mehdi Bouhaddou, Danielle L Schmitt

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Kasey ParksDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095.ORCID 0009-0001-1256-6402
Arnav JhawarDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095.
Alexia AndrikopoulosDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095.ORCID 0009-0003-9168-7820
Declan M WintersDepartment of Human Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095.ORCID 0009-0009-1100-596X
Teagan S DeanDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095.
Edmund D KapelczakDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, 90095.ORCID 0000-0002-6305-0983
Tara TeSlaaDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, 90095.ORCID 0000-0002-0504-0998
Mehdi BouhaddouDepartment of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, CA 90095.ORCID 0000-0002-9526-1427
Danielle L SchmittDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095.ORCID 0000-0002-7343-2125

Funding

UCLA NIGMS T32 Program AssessmentT32GM007185 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TORRES, JORGE · 1985 to 2020
$21.7M
Research Training in Cell and Molecular BiologyT32GM145388 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Jorge Torres · 2022 to 2026
$5.2M
Imaging Spatiotemporal Regulation of Acetyl-CoADP2GM154012 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Danielle Lynanne Schmitt · 2023 to 2026
$2.2M
Viral Biosensors of Host Post-Translational ModificationsR35GM160071 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Mehdi Bouhaddou · 2025 to 2026
$842k
NIGMS NIH HHS DP2 GM154012NIGMS NIH HHS R35 GM160071NIGMS NIH HHS T32 GM007185NIGMS NIH HHS T32 GM145388
6 · The paper itself

Abstract

AMP-activated protein kinase (AMPK) is a central regulator of cellular energy homeostasis, with over 100 identified downstream targets throughout the cell. In response to cellular stress, including energetic stress, AMPK is activated via binding of AMP and phosphorylation by upstream kinases, including liver kinase B1 (LKB1) and calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2). We and others have found that the activation of AMPK in response to cellular stress has distinct subcellular mechanisms, indicating compartmentalized regulation of AMPK signaling. Although oxidative stress is known to stimulate AMPK activity, how AMPK is spatially regulated by oxidative stress is underexplored. Using a single-fluorophore excitation-ratiometric AMPK activity reporter (ExRai AMPKAR), we find that oxidative stress induced by hydrogen peroxide (H

Identifiers

PMID41332539
PMCPMC12667911

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.