ArticleVirology journal2025
Generation and characterization of an ovine cell line derived from peripheral blood and its potential use in the study of livestock and zoonotic viral infections.
Article in Virology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
backgroundMesenchymal stromal cells (MSCs) are a population of undifferentiated, non-hematopoietic fibroblast-like cells isolated from several tissues with multipotent differentiation capacity in vitro. This study focused on the establishment and characterization of a mesenchymal stromal cell line derived from ovine peripheral blood mononuclear cells (PBMCs).
methodsMSCs were isolated from ovine blood and used to establish a continuous cell line. Several assays were performed to characterize this cell line and confirm its mesenchymal origin. First, cells were characterized by flow cytometry (FACS) using monoclonal antibodies specific for MSCs and hematopoietic non-stromal cell surface antigens. Second, real-time quantitative PCR (RT-qPCR) was performed using gene primer pairs specific for hematopoietic and mesenchymal cell surface markers. Third, we evaluated the potential of this cell line to differentiate in vitro into other lineages such as osteoblasts and neurons, which was confirmed by specific staining with Alizarin Red (AR), RT-qPCR and immunofluorescence (IFA) assays. Finally, we tested the susceptibility of this ovine cell line to diverse livestock, animal and human viruses.
resultsFACS and RT-qPCR analyses revealed that the ovine cell line expressed mesenchymal markers, and was negative for hematopoietic markers. In addition, our ovine cell line was able to differentiate into osteoblasts and neurons, as we observed via quantitative analyses of AR staining, RT-qPCR and IFA. Importantly, the ovine cell line was susceptible to infection by all the viruses tested, as confirmed by IFA and viral growth kinetics assays.
conclusionIn this work, we isolated MSCs from ovine peripheral blood and established a continuous ovine cell line. The thorough characterization conducted as well as the osteogenic and neuronal differentiation capacity of these cells confirmed that the ovine cell line had a mesenchymal stromal origin. Interestingly, the newly established ovine cell line was permissive to all viruses studied in this work, suggesting its potential as a valuable tool for isolating and characterizing viruses and studying virus-host interactions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.