Evidence map›Paper›PMID 41331662›Full record

ArticleBMC research notes2025

Enhancement of sorghum biomass hydrolysis by addition of amylases to an ionic liquid-based deconstruction process.

Md Tahmid Islam, Venkataramana R Pidatala, Edward E K Baidoo, Paul Wolski, Hemant Choudhary, Daniel H Putnam, Robert Hutmacher, Jackie Atim, Corinne D Scown, Henrik V Scheller and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Md Tahmid IslamJoint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, 94608, USA.
Venkataramana R PidatalaJoint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, 94608, USA.
Edward E K BaidooJoint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, 94608, USA.
Paul WolskiJoint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, 94608, USA.
Hemant ChoudharyJoint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, 94608, USA.
Daniel H PutnamJoint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, 94608, USA.
Robert HutmacherJoint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, 94608, USA.
Jackie AtimJoint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, 94608, USA.
Corinne D ScownJoint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, 94608, USA.
Henrik V SchellerJoint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, 94608, USA.
Blake A SimmonsJoint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, 94608, USA.
John M GladdenJoint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, 94608, USA.
Alberto RodriguezJoint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA, 94608, USA. alberto@lbl.gov.

Funding

Biological and Environmental Research DE-AC02-05CH11231
6 · The paper itself

Abstract

objectiveConversion of lignocellulosic biomass to renewable biofuels and bio-based chemicals at scale holds great promise for increasing domestic supplies of energy and alternatives to petrochemicals but is contingent upon improvements in biomass deconstruction. Here we report a method to maximize the enzymatic hydrolysis of whole plant samples containing fibrous and non-fibrous carbohydrates in sorghum, a naturally drought-tolerant high-yielding bioenergy feedstock. This process involves the addition of amylases to ionic liquid-pretreated biomass in conjunction with traditionally employed cellulase and hemicellulase cocktails.

resultsWe found that amylases are compatible with cholinium-based ionic liquids and act synergistically with other saccharolytic enzymes to deconstruct sorghum biomass. By using mixtures of sorghum grain (high starch content) and stover (low starch content), we show that amylases can produce up to 4-fold higher glucose yields from biomass with a higher starch content but even samples containing only stover can benefit from amylase addition. Total monomeric sugar concentrations of 70 and 153 g/L were obtained from stover or grain biomass, respectively, when performing a reaction with 20% solids in presence of amylases. This study presents new possibilities for process intensification during the deconstruction of starch-containing bioenergy feedstocks.

Indexed as

AmylasesBiomassIonic LiquidsSorghumBiofuelsCellulaseGlucoseGlycoside HydrolasesHydrolysisLigninStarchAmylasesBiofuelsCellulaseGlucoseGlycoside HydrolaseshemicellulaseIonic LiquidsLigninlignocelluloseStarchCholinium lysinateEnzymatic saccharificationPretreatmentProcess intensificationStarch

Identifiers

PMID41331662
PMCPMC12777037

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.