Evidence map›Paper›PMID 41331597›Full record

ArticleMicrobial cell factories2025

Multi-omics analysis reveals the carbohydrate-active enzyme repertoire involved in degrading complex arabinoxylans in Penicillium parvum 4-14.

Liangkun Long, Jiarong Zhu, Yijia Teng, Qunying Lin, Jing Wang, Shaojun Ding

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Article in Microbial cell factories, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Liangkun Long *State Key Laboratory for Development and Utilization of Forest Food Resources, Nanjing Forestry University, Nanjing, 210037, China. longlk602@njfu.edu.cn.ORCID http://orcid.org/0000-0001-7302-6330
Jiarong Zhu *Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.ORCID http://orcid.org/0009-0000-4109-1234
Yijia TengJiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.ORCID http://orcid.org/0009-0001-5604-8463
Qunying LinNanjing Institute for the Comprehensive Utilization of Wild Plants, Nanjing, 211111, China.ORCID http://orcid.org/0000-0002-5134-8002
Jing WangJiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.ORCID http://orcid.org/0000-0001-9163-695X
Shaojun DingState Key Laboratory for Development and Utilization of Forest Food Resources, Nanjing Forestry University, Nanjing, 210037, China.ORCID http://orcid.org/0000-0002-8359-9252

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCorn bran arabinoxylan (CBAX) is one of the most structurally complex xylans in nature. The bioconversion of CBAX into value-added products remains challenging because the substrate is resistant to pure xylanases and commercial enzyme cocktails. The carbohydrate-active enzymes (CAZymes) of Penicillium parvum 4-14 have been shown to efficiently hydrolyze CBAX. This study aimed to investigate the expression patterns and functional roles of CAZymes involved in the degradation of complex arabinoxylans in the fungus using transcriptomic and proteomic technologies.

resultsP. parvum 4-14 grew on CBAX and corn cob arabinoxylan (CCAX) with different substitution patterns and produced secretomes with varied compositions. The CBAX- or CCAX-induced fungal secretomes showed similar ratios (76.2% and 75.1%) on monosaccharide release from CBAX, but the former has a 12% higher ratio on monosaccharide release from CCAX than the latter. Integrated transcriptomic and proteomic analyses revealed distinct patterns of functional gene expression and CAZyme secretion in P. parvum cells induced by the two types of arabinoxylans, implying that the fungus has a complex regulatory system for CAZyme synthesis. A total of 26 CAZymes were inferred to be involved in the degradation of CBAX on the basis of multi-omics data and substrate structures. At the same fungal growth stage (48 h), 24 of these 26 CAZyme showed 0.42- to 5.74-fold higher gene transcription levels under CBAX culture than under CCAX culture.

conclusionsDifferent sources of arabinoxylans significantly affect the production of extracellular CAZymes in P. parvum. These findings are valuable for understanding the key CBAX-degrading enzymes and engineering tailored enzyme systems to valorize complex hemicelluloses.

Indexed as

PenicilliumXylansFungal ProteinsMultiomicsProteomicsTranscriptomeZea maysarabinoxylanFungal ProteinsXylansCereal branEnzymatic hydrolysisHighly branched arabinoxylanMulti-omics analysisPenicillium parvum

Identifiers

PMID41331597
PMCPMC12777493

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