Evidence map›Paper›PMID 41331498›Full record

ArticleMicrobiome2025

L. johnsonii alleviates methamphetamine craving via the metabolism of tyrosine.

Ran Zhang, Zhihao Cheng, Dongsheng Liu, Qin Shao, Wei Sheng, Hui Xu, Peng Xu, Youmei Wang, Jiye Aa, Guangji Wang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiome, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Strain-resolved biology ofFrontiers in veterinary science · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ran Zhang *Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China.
Zhihao Cheng *Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China.
Dongsheng LiuKey Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China.
Qin ShaoKey Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China.
Wei ShengKey Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China.
Hui XuChina National Narcotics Control Commission - China Pharmaceutical University Joint, Laboratory On Key Technologies of Narcotics Control, China Pharmaceutical University, Nanjing, 210009, China.
Peng XuKey Laboratory of Drug Monitoring and Control, Drug Intelligence and Forensic Center, Ministry of Public Security, Beijing, 100193, China.
Youmei WangKey Laboratory of Drug Monitoring and Control, Drug Intelligence and Forensic Center, Ministry of Public Security, Beijing, 100193, China.
Jiye AaKey Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China.
Guangji WangKey Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China. guangjiwang@hotmail.com.
Yuan XieKey Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China. yuanxie@cpu.edu.cn.

Funding

Leading technology foundation research project of Jiangsu province BK20192005National Key Research and Development Program of China 2018YFC0807403the CAMS Innovation Fund for Medical Sciences 2021-I2M-5-011the National Natural Science Foundation of China 81872932the Six Talent Peaks Project in Jiangsu Province SWYY-061
6 · The paper itself

Abstract

backgroundMethamphetamine (MA) abuse is a global public problem and methamphetamine addiction lacks of effective treatment. The gut microbes, involved in the gut-brain axis, remotely regulate methamphetamine addiction.

resultsIn this study, we identified that Lactobacillus johnsonii (L. johnsonii) is involved in the metabolism of tyrosine. MA addiction disrupts the balance of gut microbes, reducing the abundance of L. johnsonii, destroying intestinal barrier integrity, and increasing the tyrosine level. The outbreak of tyrosine promotes a greater craving for MA in mice since it transfers from the intestine to VTA and NAc to promote microglia immune reactivity, which reduces energy supply to neurons and decreases presynaptic mitochondria in dopaminergic neurons. As a consequence, the dopaminergic presynaptic membrane reuptake process based on vesicular transport is affected, leading to maintain of dopamine in the synaptic cleft acting on the postsynaptic membrane with synaptic plasticity changes, resulting in MA craving. L. johnsonii transplantation rescues mice from MA craving by promoting the metabolism of tyrosine in intestine and improves the neuronal function in NAc and VTA.

conclusionsOur results reveal that MA addiction disrupts gut microbiota homeostasis and promotes tyrosine-mediated dopamine system dysfunction. L. johnsonii transplantation is recommended for the treatment of methamphetamine craving.

Indexed as

Amphetamine-Related DisordersCravingMethamphetamineTyrosineAnimalsDopamineDopaminergic NeuronsGastrointestinal MicrobiomeMaleMiceMice, Inbred C57BLNucleus AccumbensVentral Tegmental AreaDopamineMethamphetamineTyrosineAddictionGut microbiotaLactobacillus johnsoniiMethamphetamineNeuroinflammationTyrosine

Identifiers

PMID41331498
PMCPMC12776994

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.