ArticleNature methods2026
CaBLAM: a high-contrast bioluminescent Ca
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Article in Nature methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19citing papers in PubMed
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Who cites it
19 citing papers in PubMed.
- Noninvasive methods to monitor dynamic single-cell events.Trends in cell biology · 2026Review
- Light-activated tetanus neurotoxin for conditional proteolysis and inducible synaptic inhibition in vivo.Nature methods · 2026Article
- CRISPR Gene Tagging for Illuminating Endogenous Protein Dynamics.International journal of molecular sciences · 2026Review
- A bioluminescent activity dependent platform, BLADe, for converting intracellular activity to photoreceptor activation.Scientific reports · 2026Article
- All-in-one, Cas13d-based cell-specific gene knockdown system for zebrafish.bioRxiv : the preprint server for biology · 2026Article
- Contributions of Zebrafish to Our Understanding of the Function and Regulation of Sleep.Current sleep medicine reports · 2026Review
- CaBLAM: a high-contrast bioluminescent CaNature methods · 2026Article
- Control of Synaptic Communication through Molecularly Engineered Bioluminescence Light Emission and Sensing.bioRxiv : the preprint server for biology · 2025Article
- A Bioluminescent Activity Dependent (BLADe) Platform for Converting Intracellular Activity to Photoreceptor Activation.bioRxiv : the preprint server for biology · 2025Article
- An Enhanced Red Bioluminescent Indicator for Responsive Detection of Physiological Calcium Dynamics in Cells and Mice.ACS sensors · 2025Article
- Presymptomatic targeted circuit manipulation for ameliorating Huntington's disease pathogenesis.iScience · 2025Article
- Quantitative approaches for studying G protein-coupled receptor signalling and pharmacology.Journal of cell science · 2025Review
- Presymptomatic Targeted Circuit Manipulation for Ameliorating Huntington's Disease Pathogenesis.bioRxiv : the preprint server for biology · 2024Article
- Toward a brighter constellation: multiorgan neuroimaging of neural and vascular dynamics in the spinal cord and brain.Neurophotonics · 2024Article
- Engineering luminopsins with improved coupling efficiencies.Neurophotonics · 2024Article
- Method for optimizing imaging parameters to record neuronal and cellular activity at depth with bioluminescence.Neurophotonics · 2024Article
- Efficient opto- and chemogenetic control in a single molecule driven by FRET-modified bioluminescence.Neurophotonics · 2024Article
- Towards a Brighter Constellation: Multi-Organ Neuroimaging of Neural and Vascular Dynamics in the Spinal Cord and Brain.bioRxiv : the preprint server for biology · 2023Article
- Engineering luminopsins with improved coupling efficiencies.bioRxiv : the preprint server for biology · 2023Article
Corrections and comments
- Update of
Authors and funding
34 authors.
Gerard G Lambert *Department of Neurosciences, University of California San Diego School of Medicine, La Jolla, CA, USA.
Emmanuel L Crespo *College of Medicine, Central Michigan University, Mt. Pleasant, MI, USA.
Jeremy Murphy *Robert J. and Nancy D. Carney Institute for Brain Science and Department of Neuroscience, Brown University, Providence, RI, USA.ORCID http://orcid.org/0000-0001-5595-8548
Kevin L TurnerRobert J. and Nancy D. Carney Institute for Brain Science and Department of Neuroscience, Brown University, Providence, RI, USA.ORCID http://orcid.org/0000-0002-3044-7079
Emily GershowitzDepartment of Neuroscience, New York University Grossman School of Medicine, New York, NY, USA.
Michaela CunninghamRobert J. and Nancy D. Carney Institute for Brain Science and Department of Neuroscience, Brown University, Providence, RI, USA.ORCID http://orcid.org/0009-0007-6475-0379
Daniela BoassaDepartment of Neurosciences, University of California San Diego School of Medicine, La Jolla, CA, USA.ORCID http://orcid.org/0000-0003-4631-4139
Selena LuongUniversity of California San Diego, La Jolla, CA, USA.
Dmitrijs CelinskisRobert J. and Nancy D. Carney Institute for Brain Science and Department of Neuroscience, Brown University, Providence, RI, USA.
Justine J AllenRobert J. and Nancy D. Carney Institute for Brain Science and Department of Neuroscience, Brown University, Providence, RI, USA.ORCID http://orcid.org/0000-0001-9974-4226
Stephanie VennCollege of Medicine, Central Michigan University, Mt. Pleasant, MI, USA.
Yunlu ZhuDepartment of Neuroscience, New York University Grossman School of Medicine, New York, NY, USA.
Mürsel KaradasDepartment of Neuroscience, New York University Grossman School of Medicine, New York, NY, USA.
Jiakun ChenDepartment of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Roberta MariscaInstitute of Neuronal Cell Biology, Technical University of Munich, Munich, Germany.
Hannah GelnawDepartment of Neuroscience, New York University Grossman School of Medicine, New York, NY, USA.ORCID http://orcid.org/0000-0002-7970-796X
Daniel K NguyenUniversity of California San Diego, La Jolla, CA, USA.
Junru HuNational Center for Microscopy and Imaging Research, University of California San Diego, La Jolla, CA, USA.
Brittany N SprecherDepartment of Neurosciences, University of California San Diego School of Medicine, La Jolla, CA, USA.ORCID http://orcid.org/0000-0001-5032-3834
Maya O TreeCollege of Medicine, Central Michigan University, Mt. Pleasant, MI, USA.
Richard OrcuttDepartment of Neurosciences, University of California San Diego School of Medicine, La Jolla, CA, USA.
Daniel HeydariDepartment of Neurosciences, University of California San Diego School of Medicine, La Jolla, CA, USA.
Aidan B BellUniversity of California San Diego, La Jolla, CA, USA.
Albertina Torreblanca-ZancaDepartment of Neurosciences, University of California San Diego School of Medicine, La Jolla, CA, USA.ORCID http://orcid.org/0000-0002-1016-6093
Ali HakimiScintillon Institute, San Diego, CA, USA.
Tim CzopkaInstitute of Neuronal Cell Biology, Technical University of Munich, Munich, Germany.
Shy ShohamDepartment of Neuroscience, New York University Grossman School of Medicine, New York, NY, USA.
Katherine I NagelDepartment of Neuroscience, New York University Grossman School of Medicine, New York, NY, USA.
David SchoppikDepartment of Neuroscience, New York University Grossman School of Medicine, New York, NY, USA.ORCID http://orcid.org/0000-0001-7969-9632
Arturo AndradeRobert J. and Nancy D. Carney Institute for Brain Science and Department of Neuroscience, Brown University, Providence, RI, USA.
Diane LipscombeRobert J. and Nancy D. Carney Institute for Brain Science and Department of Neuroscience, Brown University, Providence, RI, USA.
Christopher I MooreRobert J. and Nancy D. Carney Institute for Brain Science and Department of Neuroscience, Brown University, Providence, RI, USA.ORCID http://orcid.org/0000-0003-4534-1602
Ute HochgeschwenderCollege of Medicine, Central Michigan University, Mt. Pleasant, MI, USA.
Nathan C ShanerDepartment of Neurosciences, University of California San Diego School of Medicine, La Jolla, CA, USA. ncshaner@health.ucsd.edu.ORCID http://orcid.org/0000-0002-0148-0769
Funding
Selective Control of Synaptically-Connected Circuit Elements by Interluminescence - Diversity Supplement SILVAGNOLIR01NS120832 · NINDS · CENTRAL MICHIGAN UNIVERSITY · PI HOCHGESCHWENDER, UTE H, MOORE, CHRISTOPHER I · 2021 to 2023
$3.4MBioLuminescent OptoGenetics (BL-OG): A Novel and Versatile Strategy for NeuromodulationU01NS099709 · NINDS · CENTRAL MICHIGAN UNIVERSITY · PI HOCHGESCHWENDER, UTE H, MOORE, CHRISTOPHER I · 2016 to 2018
$2.4MNew tools for quantitative non-invasive recording of biochemical signalsR01CA279813 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jennifer Prescher, Nathan Christopher Shaner · 2024 to 2026
$2.0MCaV2.2 splice variants in the hippocampus: function and pharmacologyR01MH124811 · NIMH · BROWN UNIVERSITY · PI Arturo Andrade · 2022 to 2026
$1.9MDeterminants of extraocular motor neuron subtype diversityR01EY035691 · NEI · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI David Schoppik · 2024 to 2026
$1.5MProbes for Luminescence-based Superresolution MicroscopyR01GM121944 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SHANER, NATHAN CHRISTOPHER · 2017 to 2020
$1.3MTransformative Optical Imaging of Brain & Behavior in Navigating Genetic SpeciesR34DA059500 · NIDA · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI NAGEL, KATHERINE, SCHOPPIK, DAVID · 2024 to 2025
$752kAn Orthogonal Bioluminescent Platform For Multiplexed Imaging And ControlR21EY036659 · NEI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jennifer Prescher, Nathan Christopher Shaner · 2024 to 2026
$605kHighly specific control of neurons with photoswitchable bioluminescent optogenetics.R21EY030716 · NEI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SHANER, NATHAN CHRISTOPHER · 2019 to 2019
$486kGenetically Encoded Light-Production and Light-Sensing for Neuronal ManipulationR21MH101525 · NIMH · DUKE UNIVERSITY · PI HOCHGESCHWENDER, UTE H · 2013 to 2015
$434kDevelopment and molecular phenotypes of zebrafish astrocytesR21NS115437 · NINDS · OREGON HEALTH & SCIENCE UNIVERSITY · PI MONK, KELLY R · 2020 to 2020
$424kCircadian Control of the VTA: Neural and BBB DynamicsF32NS134617 · NINDS · BROWN UNIVERSITY · PI TURNER, KEVIN LAWRENCE · 2024 to 2025
$149kBrown University (Brown) OVPR seed awardNCI NIH HHS R01 CA279813NEI NIH HHS R01 EY035691NEI NIH HHS R21 EY030716NEI NIH HHS R21 EY036659NIDA NIH HHS R34 DA059500NIGMS NIH HHS R01 GM121944NIMH NIH HHS R01 MH124811NIMH NIH HHS R21 MH101525NINDS NIH HHS F32 NS134617NINDS NIH HHS F99 NS129170NINDS NIH HHS R01 NS120832NINDS NIH HHS R21 NS115437NINDS NIH HHS U01 NS099709NSF | BIO | Division of Biological Infrastructure (DBI) 1707352NSF | BIO | Division of Biological Infrastructure (DBI) 2208914NSF | ENG/OAD | Division of Chemical, Bioengineering, Environmental, and Transport Systems (CBET) 1464686Paul G. Allen Family Foundation Allen Discovery Center for Neurobiology in Changing EnvironmentsU.S. Department of Health & Human Services | National Institutes of Health (NIH) F32NS134617U.S. Department of Health & Human Services | National Institutes of Health (NIH) R01CA279813U.S. Department of Health & Human Services | National Institutes of Health (NIH) R01GM121944U.S. Department of Health & Human Services | National Institutes of Health (NIH) R01MH124811U.S. Department of Health & Human Services | National Institutes of Health (NIH) R01NS120832U.S. Department of Health & Human Services | National Institutes of Health (NIH) R21EY030716U.S. Department of Health & Human Services | National Institutes of Health (NIH) R21EY036659U.S. Department of Health & Human Services | National Institutes of Health (NIH) R21NS115437U.S. Department of Health & Human Services | National Institutes of Health (NIH) R34DA059500U.S. Department of Health & Human Services | National Institutes of Health (NIH) U01NS099709
Abstract
Monitoring intracellular calcium is central to understanding cell signaling across nearly all cell types and organisms. Fluorescent genetically encoded calcium indicators (GECIs) remain the standard tools for in vivo calcium imaging, but require intense excitation light, leading to photobleaching, background autofluorescence and phototoxicity. Bioluminescent GECIs, which generate light enzymatically, eliminate these artifacts but have been constrained by low dynamic range and suboptimal calcium affinities. Here we show that CaBLAM ('calcium bioluminescence activity monitor'), an engineered bioluminescent calcium indicator, achieves an order-of-magnitude improvement in signal contrast and a tunable affinity matched to physiological cytosolic calcium. CaBLAM enables single-cell and subcellular activity imaging at video frame rates in cultured neurons and sustained imaging over hours in awake, behaving animals. These capabilities establish CaBLAM as a robust and general alternative to fluorescent GECIs, extending calcium imaging to regimes where excitation light is undesirable or infeasible.
Indexed as
CalciumLuciferasesLuminescent MeasurementsAnimalsCalcium SignalingHEK293 CellsHumansMiceNeuronsCalciumLuciferases
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