Evidence map›Paper›PMID 41331071›Full record

ArticleMicrosystems & nanoengineering2025

Oil-sealed RGD-modified alginate hydrogel microwell array for analysis of single-cell-derived extracellular vesicles and particles.

Chisaki Yamagata, Yuto Hamazaki, Tomoharu Nakazato, Shun Itai, Mai Honjo, Mami Kato, Yuta Kurashina, Makoto Asai, Ayuko Hoshino, Hiroaki Onoe

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microsystems & nanoengineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Photo-guided azopolymer hydrogel actuators.Light, science & applications · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Chisaki YamagataSchool of Integrated Design Engineering, Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-Ku, Yokohama, 223-8522, Japan.
Yuto HamazakiSchool of Life Science and Technology, Institute of Science Tokyo, Nagatsuta-cho 4259, Midori-ku, Yokohama, 226-8501, Japan.
Tomoharu NakazatoSchool of Integrated Design Engineering, Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-Ku, Yokohama, 223-8522, Japan.
Shun ItaiSchool of Integrated Design Engineering, Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-Ku, Yokohama, 223-8522, Japan.
Mai HonjoResearch Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan.
Mami KatoResearch Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan.
Yuta KurashinaDivision of Advanced Mechanical Systems Engineering, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo, 184-8588, Japan.
Makoto AsaiKeio University Global Research Institute, Keio University, 2-15-45 Mita, Minato-ku, Tokyo, 108-8345, Japan.
Ayuko HoshinoSchool of Life Science and Technology, Institute of Science Tokyo, Nagatsuta-cho 4259, Midori-ku, Yokohama, 226-8501, Japan. aynhoshino@g.ecc.u-tokyo.ac.jp.
Hiroaki OnoeSchool of Integrated Design Engineering, Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-Ku, Yokohama, 223-8522, Japan. onoe@mech.keio.ac.jp.ORCID http://orcid.org/0000-0003-0048-1580

Funding

Japan Agency for Medical Research and Development (AMED) 22zf0127009h000Japan Agency for Medical Research and Development (AMED) 24zf0127012Keio University (Keio) Research Project Keio 2040 (Creativity Initiative)MEXT | Japan Science and Technology Agency (JST) JPMJFR216BMEXT | Japan Science and Technology Agency (JST) JPMJMS2292MEXT | Japan Science and Technology Agency (JST) JPMJSP2123MEXT | Japan Society for the Promotion of Science (JSPS) 24KJ0956MEXT | Japan Society for the Promotion of Science (JSPS) 24KJ0984MEXT | Japan Society for the Promotion of Science (JSPS) JP20H05904MEXT | Japan Society for the Promotion of Science (JSPS) JP25H01066MEXT | Japan Society for the Promotion of Science (JSPS) JP25KJ0103
6 · The paper itself

Abstract

We propose an oil-sealed, arginine-glycine-aspartic (RGD) -modified alginate hydrogel microwell array for the analysis of single-cell-derived extracellular vesicles and particles (EVPs) secreted by adherent cells cultured in enclosed spaces. Taking advantage of the mesh structure of alginate hydrogel, we developed a microwell array with size-selective permeability that allows nutrients to pass through the hydrogel while preventing EVPs from doing so. Continuous single-cell culture in sealed microwells (>19 days) has been achieved, while retaining the EVPs inside the microwells. The single-cell-derived EVPs were collected from sealed microwells using a glass capillary to analyze surface membrane proteins. We believe that our oil-sealed RGD-modified alginate hydrogel microwell array will contribute to revealing the heterogeneity of cells, thereby advancing our understanding of the mechanisms of various diseases.

Identifiers

PMID41331071
PMCPMC12672703

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.