ArticleRNA (New York, N.Y.)2026
MUTACLASH: identifying functional small RNA target sites using crosslinking-induced mutations.
Article in RNA (New York, N.Y.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Small RNAs play essential roles in gene regulation across diverse biological processes. Crosslinking, ligation, and sequencing of hybrids (CLASH) experiments have revealed that PIWI and Argonaute proteins can each bind a wide range of mRNA targets with distinct base-pairing rules, raising questions about the flexibility and functional relevance of these interactions. Given that crosslinking-induced mutations (CIMs) provide single-nucleotide resolution molecular footprints of RNA-binding proteins, we developed MUTACLASH, a bioinformatics tool for systematically analyzing CIMs in CLASH data sets. Our analyses indicate that CIMs function as molecular footprints of Argonaute binding on target mRNAs. Specifically, for
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