Evidence map›Paper›PMID 41328409›Full record

ArticleSynthetic and systems biotechnology2026

Defect-complementation homologous recombination: A novel strategy for precise genome engineering of virulent phages.

Hailin Zhang, Yueyue Song, Wenyue Liu, Xiaoqing Zheng, Xiaodong An, Chao Li, Weihua Chen, Hailong Wang, Yuran Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Synthetic and systems biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hailin ZhangSchool of Life Sciences, Jining Medical University, No. 669 Xueyuan Road, Donggang District, Rizhao, Shandong Province, 276826, China.
Yueyue SongSchool of Life Sciences, Jining Medical University, No. 669 Xueyuan Road, Donggang District, Rizhao, Shandong Province, 276826, China.
Wenyue LiuSchool of Life Sciences, Jining Medical University, No. 669 Xueyuan Road, Donggang District, Rizhao, Shandong Province, 276826, China.
Xiaoqing ZhengSchool of Life Sciences, Jining Medical University, No. 669 Xueyuan Road, Donggang District, Rizhao, Shandong Province, 276826, China.
Xiaodong AnRizhao Center for Disease Control and Prevention, No. 136, Beijing Road, Donggang District, Rizhao, Shandong Province, 276826, China.
Chao LiRizhao Center for Disease Control and Prevention, No. 136, Beijing Road, Donggang District, Rizhao, Shandong Province, 276826, China.
Weihua ChenSchool of Life Sciences, Jining Medical University, No. 669 Xueyuan Road, Donggang District, Rizhao, Shandong Province, 276826, China.
Hailong WangState Key Laboratory of Microbial Technology, Institute of Microbial Technology, Helmholtz International Lab for Anti-infectives, Shandong University-Helmholtz Institute of Biotechnology, Shandong University, Qingdao, Shandong, 266237, China.
Yuran ZhangSchool of Life Sciences, Jining Medical University, No. 669 Xueyuan Road, Donggang District, Rizhao, Shandong Province, 276826, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Engineered bacteriophages (phages) have been developed to overcome the limitations of natural phage therapies and serve as precision-targeted agents against drug-resistant bacterial infections. However, their application has been constrained by the low efficiency of existing genome-editing tools, largely because of the absence of effective selection markers. This study proposed a novel strategy, termed defect-complementation homologous recombination (DCHR), for precise phage genome editing. In this approach, CRISPR-Cas9 cleaves a donor plasmid in host cells to release a linear donor template carrying homology arms, an essential phage gene used as a selection marker, and two

Indexed as

Cre-loxGenetic complementationHomologous recombinationPhage genetic manipulationPhage genome engineering

Identifiers

PMID41328409
PMCPMC12664982

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.