Evidence map›Paper›PMID 41328230›Full record

ArticleJournal of advanced veterinary and animal research2025

Establishment of Vero cell-adapted thermostable candidate Newcastle Disease vaccine strain.

Md Humayun Kabir, Md Amirul Hasan, Yu Miyaoka, Makiko Yamaguchi, Chisaki Kadota, Dany Shoham, Harumi Murakami, Kazuaki Takehara

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of advanced veterinary and animal research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Md Humayun KabirLaboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
Md Amirul HasanLaboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
Yu MiyaokaLaboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
Makiko YamaguchiLaboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
Chisaki KadotaLaboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
Dany ShohamBegin-Sadat Center for Strategic Studies, Bar-Ilan University, Ramat Gan, Israel.
Harumi MurakamiLaboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
Kazuaki TakeharaLaboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: The current study aims to determine a thermostable Avian Orthoavulavirus 1 (AOaV-1) or Newcastle disease (ND) vaccine candidate strain isolated from a duck and its adaptation to Vero cells. Material and Methods: From field isolates of AOaV-1 in Japan, avirulent APMV/northern pintail/Japan/Aomori/2003 (dk-Amori/03, AOaV-1-261) was selected for its excellent thermostability, and the strain was heat treated at 56°C for 30 min during each passage into Vero cells to maintain thermostability and to adapt to Vero cells up to 20 passages, then named AOaV-1-Vero20. Growth curves were tested in Vero cells in comparison to the original AOaV-1-261. The intracerebral pathogenicity index (ICPI) test was done. The thermostability of the virus was checked at different temperatures. Serum neutralization test was done to check the induced levels of antibodies of the candidate vaccine strain in chicks. Sequencing of the Results: AOaV-1-Vero20 grew well on Vero cells compared to the original AOaV-1-261. The ICPI test score was 0. Minimum titer drops were found in the thermostability test. Induced, adequate levels of antibodies were measured using the serum neutralization test. Sequencing of the Conclusion: In this study, AOaV-1-Vero20 was identified as a candidate vaccine strain that is thermostable, Vero cell-adapted, and has immunogenic potential.

Indexed as

AntibodyNewcastle disease virusthermostabilityvaccineVero cell

Identifiers

PMID41328230
PMCPMC12665325

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.