ArticleStem cell research & therapy2025
Intracellular autofluorescence enables the isolation of viable, functional human muscle reserve cells with distinct Pax7 levels and stem cell states.
Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundHuman muscle reserve cells (MuRC) represent a quiescent MuSC population generated in vitro that exhibit heterogeneous Pax7 expression, with a Pax7
methodsHuman myoblasts (MB) and MuRC were analysed for AF by fluorescence microscopy and flow cytometry. Cellular metabolic composition was assessed by NADH/NADPH quantification and lipid staining. Human MuRC subpopulations were sorted by AF intensity and analysed for Pax7 expression, cell cycle re-entry, proliferation, clonal expansion, and myogenic differentiation. In vivo transplantation of MuRC-AF
resultsHuman MuRC exhibited a threefold increase in autofluorescence intensity compared to MB, with a peak at 405 nm excitation, likely linked to a 1.6-fold increase in lipid content, while NADH/NADPH levels remained comparable. Flow cytometry identified MuRC-AF
conclusionAutofluorescence enables the isolation of distinct human MuRC subpopulations. The AF
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