Evidence map›Paper›PMID 41327426›Full record

ArticleVeterinary research2025

Nephropathogenic infectious bronchitis virus-induced pyroptosis of chicken renal tubular epithelial cells via the MDA5/NF-κB/NLRP3 signalling pathway.

Bingqing Zhang, Mengbing Ding, Yizhou Zeng, Jingyan Luo, Jiaqi Li, Xiaona Gao, Ping Liu, Gaofeng Cai, Zhanhong Zheng, Xiaoquan Guo

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Article in Veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

10 authors.

Bingqing Zhang *Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China.
Mengbing Ding *Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China.
Yizhou ZengJiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China.
Jingyan LuoJiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China.
Jiaqi LiJiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China.
Xiaona GaoJiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China.
Ping LiuJiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China.
Gaofeng CaiJiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China.
Zhanhong ZhengJiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China.
Xiaoquan GuoJiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China. xqguo20720@jxau.edu.cn.ORCID http://orcid.org/0009-0008-9115-246X

Funding

China National Natural Science Foundation 32072935China National Natural Science Foundation 32360906Graduate Student Innovation Fund of Jiangxi Province YC2024-S335Natural Science Foundation of Jiangxi Province 20224BAB215056The National Key R&D Program of China 2023YFD1801100
6 · The paper itself

Abstract

MDA5 is an innate pattern recognition receptor that is involved in the recognition of various viruses. It can recognize RNA viruses, activate downstream signalling pathways, facilitate the transcription of inflammatory factors, and induce cell pyroptosis. Pyroptosis is a form of programmed cell death accompanied by the release of inflammatory factors and an inflammatory response. In this study, we hypothesize that pyroptosis is elicited by the signalling cascade subsequent to the recognition of nephropathogenic infectious bronchitis virus (NIBV) by MDA5. Thus, we infected chicken renal tubular epithelial cells with NIBV and discovered that NIBV infection induced pyroptosis and increased the mRNA levels of MDA5. Consequently, we infected primary chicken renal tubular epithelial cells with NIBV and inhibited TRAF6 expression using the exogenous inhibitor C25-140. We found that NIBV could increase lactate dehydrogenase (LDH) levels, increase the proportion of pyroptotic cells, and increase the mRNA and protein levels of the MDA5/NF-κB signalling pathway and the classical pyroptosis pathway. Here, we selected the ubiquitin ligase TRAF6, a key node in the MDA5/NF-κB signalling pathway, from molecular biological and genetic perspectives to explore the molecular mechanism of NIBV-induced pyroptosis. After the inhibitor C25-140 was used, NIBV-induced apoptosis and the activity of the MDA5/NF-κB/NLRP3 pathway were reversed. In addition, the amount of NIBV replication in the cells was reduced. In conclusion, the MDA5/NF-κB/NLRP3 signalling pathway is involved in the regulation of pyroptosis in a NIBV-infected chicken renal tubular epithelial cell model. The inhibition of this signalling pathway can alleviate NIBV-induced pyroptosis and reduce the replication of NIBV in cells, which could become one strategy for treating NIBV.

Indexed as

Avian ProteinsChickensCoronavirus InfectionsInfectious bronchitis virusInterferon-Induced Helicase, IFIH1Poultry DiseasesPyroptosisSignal TransductionAnimalsEpithelial CellsKidney TubulesNF-kappa BNLR Family, Pyrin Domain-Containing 3 ProteinAvian ProteinsInterferon-Induced Helicase, IFIH1NF-kappa BNLR Family, Pyrin Domain-Containing 3 ProteinMDA5/NF-κB/NLRP3 signalling pathwayNIBVpyroptosisrenal tubular epithelial cells

Identifiers

PMID41327426
PMCPMC12777303

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.