ArticleVeterinary research2025
PotD contributes to Streptococcus suis-induced blood-brain barrier disruption by regulating arcA transcription.
Article in Veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Pathogenicity and virulence ofVirulence · 2026Review
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Authors and funding
9 authors.
Funding
Abstract
The ABC transporter substrate-binding protein PotD plays an important role in bacteria for polyamine uptake, but its role in the pathogenicity of Streptococcus suis (S. suis) in the host is still unknown. Our study investigated the mechanism by which PotD mediates S. suis pathogenicity, especially S. suis-induced blood-brain barrier (BBB) disruption. The results showed that ΔpotD mutant significantly reduced biofilm formation and bacterial load in different tissues, including brain, blood, liver, and lung. Importantly, knockout of potD significantly improved mouse survival rate, indicating that PotD is involved in S. suis pathogenicity. The deletion of potD significantly reversed the reduction of tight junction proteins ZO-1 and Occludin in mouse brain and human cerebral microvascular endothelial cell line D3 (hCMEC/D3), and attenuated BBB disruption via Evans blue (EB) staining assay. Notably, recombinant PotD protein exhibited the disruption of BBB by downregulating ZO-1 and Occludin in hCMEC/D3 cells, demonstrating that PotD contributes to S. suis-induced BBB disruption. Furthermore, mass spectrometry analysis and pulldown assay revealed that PotD interacted with arginine deiminase (ADI) encoded by arcA and mediated arcA transcription. In addition, ΔarcA mutant attenuated BBB disruption, and recombinant ADI induced BBB disruption by downregulating ZO-1 and Occludin in hCMEC/D3 cells. Our study reveals the destructive role of the virulence factors potD and arcA of S. suis on the BBB and provides new insights into S. suis pathogenicity.
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