ArticleBMC veterinary research2025
Establishment of a TaqMan multiplex quantitative PCR method for detecting three different serovars of Glaesserella parasuis serovars 5/12, 7 and 11.
Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Glaesserella parasuis, a commensal in the upper respiratory tract of swine, can cause Glässer's disease in the stress conditions, which caused great economic loss to the swine industry. So far, 15 different serovars have been identified, and which are unevenly distributed in different countries and regions. To date, dominant serovars of 5/12, 7, and 11 have significantly increased in China. To rapidly and simultaneously detect these 3 dominant serovars, a TaqMan multiplex quantitative PCR (qPCR) detection method was developed. The study designed primers and probes based on the conserved sequences of the specific genes wcwK, funQ, and amtA for serovars 5/12, 7, and 11, respectively, the reaction and conditions for single and triplex systems were respectively optimized. A TaqMan multiplex qPCR method was successfully established that can simultaneously identify these three serovars. The specificity and sensitivity of the results indicated that the detection limit for the concentrations of the wcwK, funQ, and amtA genes was 10
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