Evidence map›Paper›PMID 41326999›Full record

ArticleBMC plant biology2025

An MYB transcription factor regulates capsaicinoid biosynthesis during pepper fruit ripening.

Mingxian Zhang, Shu Han, Ziran Wang, Zhengcong Luo, Xuewen Chen, Guining Fang, Qiaoli Xie, Minghua Deng, Junheng Lv

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Lighting up the pepper heat.The New phytologist · 2026
    Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Mingxian Zhang *Key Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China.
Shu Han *Key Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China.
Ziran Wang *Key Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China.
Zhengcong LuoKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China.
Xuewen ChenKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China.
Guining FangKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China.
Qiaoli XieCollege of Bioengineering, Chongqing University, Chongqing, 400044, China.
Minghua DengKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China. 2003023@ynau.edu.cn.
Junheng LvKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China. junhenglv@ynau.edu.cn.

Funding

National Natural Science Foundation of China 32360744Project of Yunnan Science and Technology Plan 202502AE090018，202204BI090004, 202101BD070001-005"Xingdian talent supportplan" of Yunnan Province XDYC-CYCX-2022-0034Yunnan Fundamental Research Projects 202301BD070001-016
6 · The paper itself

Abstract

backgroundPepper is a fruit, vegetable, and spice crop and is widely cultivated around the world. This study analyzed the transcriptomes and metabolomes of pepper fruits at six different developmental stages to analyze the changes in metabolites and genes during fruit development and further explore and elucidate the functions of the key transcription factors regulating capsaicinoid biosynthesis.

resultsA total of 1775 differential metabolites were identified between adjacent developmental stages. These metabolites were mainly amino acids and derivatives, flavonoids, alkaloids, and phenolic acids. The transcriptome analysis revealed 15,741 differential genes between adjacent developmental stages. The functional enrichment analysis indicated that the S3–S4 stage was related to capsaicinoid accumulation, while the S5–S6 stage was associated with fruit color change. CcMYB4 was identified and the transient silencing and overexpression analyses showed that CcMYB4 could regulate the expression of capsaicinoid biosynthetic structural genes and capsaicinoid accumulation. The yeast one-hybrid, electrophoretic mobility shift assays, and dual luciferase demonstrated that CcMYB4 could bind to the Cc4CL promoter and inhibit its expression, thereby regulating capsaicinoid accumulation.

conclusionsOur study investigates the entire developmental process of pepper fruits and identifies CcMYB4 as the regulator of capsaicinoid biosynthesis. The results from this study improve understanding about the molecular regulatory mechanisms associated with pepper fruit development and capsaicinoid biosynthesis.

Indexed as

CapsaicinCapsicumFruitPlant ProteinsTranscription FactorsGene Expression Regulation, PlantCapsaicinPlant ProteinsTranscription FactorsCapsaicinoid biosynthesisCapsicum chinenseFruit developmentMYB transcription factor

Identifiers

PMID41326999
PMCPMC12777249

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.