Evidence map›Paper›PMID 41326987›Full record

ArticleBMC microbiology2025

Genomic insights into multidrug resistant Escherichia coli from bovine mastitis in Bangladesh.

Naim Siddique, Kh Yeashir Arafat, Md Abu Ahsan Gilman, Md Morshedur Rahman, Ziban Chandra Das, Tofazzal Islam, M Nazmul Hoque

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Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Draft genome sequence ofMicrobiology resource announcements · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Naim Siddique *Molecular Biology and Bioinformatics Laboratory, Department of Gynecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.
Kh Yeashir Arafat *Molecular Biology and Bioinformatics Laboratory, Department of Gynecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.
Md Abu Ahsan Gilman *Molecular Biology and Bioinformatics Laboratory, Department of Gynecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.
Md Morshedur RahmanMolecular Biology and Bioinformatics Laboratory, Department of Gynecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.
Ziban Chandra DasMolecular Biology and Bioinformatics Laboratory, Department of Gynecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.
Tofazzal IslamInstitute of Biotechnology and Genetic Engineering, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.
M Nazmul HoqueMolecular Biology and Bioinformatics Laboratory, Department of Gynecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, 1706, Bangladesh. nazmul90@gau.edu.bd.

Funding

Ministry of Education (BANBEIS), Government of the People's Republic of Bangladesh LS20221764, duration 2023-2025
6 · The paper itself

Abstract

backgroundMastitis poses a significant threat to dairy industry and public health due to the emergence of multidrug-resistant (MDR) Escherichia coli. This study provides a genomic characterization of two MDR E. coli strains, MBBL4 and MBBL5, from bovine mastitis in Bangladesh, highlighting their evolutionary relationships, resistome, and virulome.

methodsSpecies-level identification of MBBL4 and MBBL5 was confirmed using biochemical assays, VITEK-2 system, and 16S rRNA gene sequencing. Antimicrobial susceptibility profiling was conducted to determine their resistance patterns. Whole genome sequencing (WGS) and comprehensive genomic analysis were performed for phylogenetic, comparative genomics, mobile genetic elements (MGEs), antimicrobial resistance genes (ARGs), and virulence factor genes (VFGs) analyses.

resultsBoth isolates exhibited extensive MDR patterns, showing resistance to ten antibiotics. Phylogenetic and ANI analyses showed that MBBL4 clustered with mastitis-associated and human bacteremia strains of E. coli, while MBBL5 was closely related to wildlife-associated strains, reflecting divergent evolutionary lineages. Pangenome analysis revealed an open pangenome structure, indicating high genetic diversity, with MBBL4 harboring 21 unique genes and MBBL5 possessing nine unique genes. Both genomes harbored numerous ARGs spanning over 11 antibiotic classes, and VFGs, predominantly associated with adherence and secretion systems, underscoring their extensive resistome, virulome, and adaptive potentials. Abundant MGEs (plasmids, prophages, insertion sequence elements and genomic islands) further underscored the role of horizontal gene transfer in driving resistance and virulence in these strains.

conclusionThis study highlights the zoonotic potential and adaptive capacity of MDR E. coli from bovine mastitis in Bangladesh driven by resistome, virulome, and mobile genetic elements. These findings highlight the urgent need for One Health-based genomic surveillance to mitigate MDR E. coli transmission from dairy farms to humans and the environment.

Indexed as

Drug Resistance, Multiple, BacterialEscherichia coliEscherichia coli InfectionsMastitis, BovineAnimalsAnti-Bacterial AgentsBangladeshCattleFemaleGenome, BacterialGenomicsMicrobial Sensitivity TestsPhylogenyRNA, Ribosomal, 16SVirulence FactorsWhole Genome SequencingAnti-Bacterial AgentsRNA, Ribosomal, 16SVirulence FactorsBovine mastitisEscherichia coliEvolutionMultidrug resistanceVirulence

Identifiers

PMID41326987
PMCPMC12667134

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.