Evidence map›Paper›PMID 41326839›Full record

ArticleMetabolomics : Official journal of the Metabolomic Society2025

Perfluorooctanoic acid disrupts hepatic metabolism in the developing chicken embryo.

Nikolai Scherbak, Daniel Duberg, Matilda Kråkström, Pauli Tikka, Alex M Dickens, Magnus Engwall, Matej Orešič, Tuulia Hyötyläinen

Abstract read
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In one paragraph

Article in Metabolomics : Official journal of the Metabolomic Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Nikolai ScherbakMTM Research Centre, School of Science and Technology, Örebro University, SE-701 82, Örebro, Sweden.
Daniel DubergMTM Research Centre, School of Science and Technology, Örebro University, SE-701 82, Örebro, Sweden.
Matilda KråkströmTurku Centre for Biotechnology, University of Turku and Åbo Akademi University, FI-20520, Turku, Finland.
Pauli TikkaTurku Centre for Biotechnology, University of Turku and Åbo Akademi University, FI-20520, Turku, Finland.
Alex M DickensTurku Centre for Biotechnology, University of Turku and Åbo Akademi University, FI-20520, Turku, Finland.
Magnus EngwallMTM Research Centre, School of Science and Technology, Örebro University, SE-701 82, Örebro, Sweden.
Matej OrešičTurku Centre for Biotechnology, University of Turku and Åbo Akademi University, FI-20520, Turku, Finland.
Tuulia HyötyläinenMTM Research Centre, School of Science and Technology, Örebro University, SE-701 82, Örebro, Sweden. tuulia.hyotylainen@oru.se.

Funding

HORIZON EUROPE Framework Programme 101136259Novo Nordisk Fonden NNF20OC0063971Stiftelsen för Kunskaps- och Kompetensutveckling 20220122Svenska Forskningsrådet Formas 2019-00869Vetenskapsrådet 2020-03674
6 · The paper itself

Abstract

introductionPerfluorooctanoic acid (PFOA) is a widespread environmental contaminant that interferes with multiple biological pathways, with lipid metabolism being particularly vulnerable. Early-life exposure may disrupt hepatic function during development, but the underlying mechanisms are not fully understood.

objectivesThis study investigated how in ovo exposure to PFOA affects hepatic metabolism in the developing chicken embryo, with a focus on identifying altered metabolic pathways and potential mediators of toxicity.

methodsFertilized chicken eggs (Gallus gallus domesticus) were exposed in ovo to six concentrations of PFOA (0-5 µg/g egg). Embryonic liver tissues were analysed by comprehensive metabolomic profiling using two complementary ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) platforms.

resultsWe identified 499 metabolites, including lipids, bile acids, carboxylic acids, amino acids, and phenolic compounds. PFOA exposure caused dose-dependent disturbances in lipid, bile acid, and amino acid metabolism. Notably, multiple secondary bile acids were detected and found to be strongly affected by PFOA, suggesting a central role of bile acid modulation in mediating its effects.

conclusionsIn ovo exposure to PFOA disrupts hepatic metabolism in developing chicken embryos, particularly through alterations in bile acid, lipid, and amino acid pathways. These metabolic changes may impair energy production, endocrine regulation, and organ development, with possible long-term health consequences.

Indexed as

CaprylatesFluorocarbonsLiverAnimalsBile Acids and SaltsChick EmbryoChickensChromatography, High Pressure LiquidLipid MetabolismMetabolomicsBile Acids and SaltsCaprylatesFluorocarbonsperfluorooctanoic acidAvian modelBile acidsLipidsLiver metabolismMass spectrometryPerfluorooctanoic acid

Identifiers

PMID41326839
PMCPMC12669338

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.