Evidence map›Paper›PMID 41326698›Full record

ArticleApplied microbiology and biotechnology2025

An efficient electrotransformation method for three Bacillus species.

Wei Quan, Chen-Lu Liu, Shao-Xuan Shi, Li-Jun Hao, Hai-Xia Wang, Zhi-Gang Liu, Bao-Zhu Dong, Hong-You Zhou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Wei QuanCollege of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Key Laboratory of Biopesticide Creation and Resource Utilization for Autonomous Region Higher Education Institutions, Hohhot, 010018, China.
Chen-Lu LiuCollege of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Key Laboratory of Biopesticide Creation and Resource Utilization for Autonomous Region Higher Education Institutions, Hohhot, 010018, China.
Shao-Xuan ShiCollege of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Key Laboratory of Biopesticide Creation and Resource Utilization for Autonomous Region Higher Education Institutions, Hohhot, 010018, China.
Li-Jun HaoCollege of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Key Laboratory of Biopesticide Creation and Resource Utilization for Autonomous Region Higher Education Institutions, Hohhot, 010018, China.
Hai-Xia WangInstitute of Resources, Environment and Sustainable Development, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, 010031, China.
Zhi-Gang LiuInner Mongolia Sihai Agriculture and Animal, Husbandry Technology Co., Ltd., Xilingol League, 027000, China.
Bao-Zhu DongCollege of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Key Laboratory of Biopesticide Creation and Resource Utilization for Autonomous Region Higher Education Institutions, Hohhot, 010018, China. dongbaozhu2020@imau.edu.cn.
Hong-You ZhouCollege of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Key Laboratory of Biopesticide Creation and Resource Utilization for Autonomous Region Higher Education Institutions, Hohhot, 010018, China. zhouhongyou@imau.edu.cn.

Funding

Central Government Guides Local Funding 2022ZY0060Inner Mongolia Autonomous Region Science and Technology Major Project Funding 2021ZD0002National Key Research and Discovery Program Funding 2023YFD1600701-5Oat and Buckwheat Industry Technology System Funding CARS-07-C-3
6 · The paper itself

Abstract

Bacillus strains are widely used in food fermentation and plant disease control. An efficient transformation method is crucial for genetic manipulation in these organisms. To enhance the transformation efficiency of three Bacillus strains-Bacillus amyloliquefaciens YN-J3 (B.a YN-J3), Bacillus velezensis JN-Y2 (B.v JN-Y2) and Bacillus subtilis S-16 (B.s S-16), we optimized transformation conditions using orthogonal experiments combined with response surface analysis. Additionally, we tested various cell wall agents to improve competence. Our results showed that the optimal transformation parameters for B.a YN-J3 and B.v JN-Y2 had an OD

Indexed as

BacillusBacillus amyloliquefaciensBacillus subtilisElectroporationTransformation, BacterialCell WallPlasmidsBacillus amyloliquefaciensBacillus subtilisBacillus velezensisElectroporationResponse surface methodology (RSM)Transformation

Identifiers

PMID41326698
PMCPMC12669251

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.