ArticleNature communications2025
All-optical strategies to minimize photobleaching in reversibly switchable fluorescent proteins.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Theoretical Design of All-Optical Universal Logic via Multistage FRET-STED Energy Cascades in Fluorescent Protein Nanoclusters.ACS omega · 2026Article
- A Versatile Tool to Predict and Guide RESOLFT Images Based on Photoswitching, Labelling and Optical Properties.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Photobleaching is a general hurdle of fluorescence-based techniques especially in high-resolution microscopy that relies on prolonged and complex illumination. Strategies to reduce photobleaching require chemical modifications of the cell medium, which often compromise physiological cellular conditions. Here, we outline an all-optical strategy to minimize photobleaching in reversibly switching fluorescent proteins (RSFPs), a class of probes used in super-resolution and protein-multiplexing imaging techniques. By identifying the photobleaching pathways, we develop imaging schemes to increase the number of on-off photoswitching cycles, either modulating the on-switching light or co-irradiating the RSFPs with light at longer wavelengths with respect to fluorescence excitation. We apply the optimized imaging scheme to achieve imaging multiplexing at high-spatiotemporal resolutions and to record longer time-lapse imaging of sub-cellular structures with both confocal microscopy and parallelized RESOLFT nanoscopy.
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Registered trials
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