Evidence map›Paper›PMID 41326206›Full record

ArticleJournal of medicinal chemistry2025

Probing Guanidino Pendant or Bridged Groups in Cyclic Antimicrobial Peptides Derived from Temporin L: A Strategy to Improve Efficacy against Gram-Negative Bacteria.

Rosa Bellavita, Ida Boccino, Maria Rosa Loffredo, Sara Palladino, Floriana Cappiello, Carlo Vetrano, Eeva Tortellini, Vincenzo Mazzarella, Salvatore Di Maro, Stefania Galdiero and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of medicinal chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Rosa BellavitaDepartment of Pharmacy, University of Naples Federico II, via Domenico Montesano 49, 80131 Naples, Italy.ORCID 0000-0003-2163-5163
Ida BoccinoDepartment of Pharmacy, University of Naples Federico II, via Domenico Montesano 49, 80131 Naples, Italy.ORCID 0009-0002-6662-007X
Maria Rosa LoffredoDepartment of Biochemical Sciences, Laboratory Affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Sapienza University of Rome, p.le Aldo Moro 5, Rome 00185, Italy.
Sara PalladinoDepartment of Pharmacy, University of Naples Federico II, via Domenico Montesano 49, 80131 Naples, Italy.ORCID 0009-0003-6255-8800
Floriana CappielloDepartment of Biochemical Sciences, Laboratory Affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Sapienza University of Rome, p.le Aldo Moro 5, Rome 00185, Italy.
Carlo VetranoDepartment of Biochemical Sciences, Laboratory Affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Sapienza University of Rome, p.le Aldo Moro 5, Rome 00185, Italy.
Eeva TortelliniDepartment of Biochemical Sciences, Laboratory Affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Sapienza University of Rome, p.le Aldo Moro 5, Rome 00185, Italy.
Vincenzo MazzarellaDiSTABiF, University of Campania Luigi Vanvitelli, via Vivaldi 43, 81100 Caserta, Italy.ORCID 0009-0005-8258-9713
Salvatore Di MaroDiSTABiF, University of Campania Luigi Vanvitelli, via Vivaldi 43, 81100 Caserta, Italy.ORCID 0000-0002-9286-4433
Stefania GaldieroDepartment of Pharmacy, University of Naples Federico II, via Domenico Montesano 49, 80131 Naples, Italy.ORCID 0000-0002-7849-7024
Bruno CasciaroDepartment of Biochemical Sciences, Laboratory Affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Sapienza University of Rome, p.le Aldo Moro 5, Rome 00185, Italy.ORCID 0000-0003-1295-1084
Paolo GriecoDepartment of Pharmacy, University of Naples Federico II, via Domenico Montesano 49, 80131 Naples, Italy.ORCID 0000-0002-6854-8123
Maria Luisa MangoniDepartment of Biochemical Sciences, Laboratory Affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Sapienza University of Rome, p.le Aldo Moro 5, Rome 00185, Italy.ORCID 0000-0002-5991-5868
Francesco MerlinoDepartment of Pharmacy, University of Naples Federico II, via Domenico Montesano 49, 80131 Naples, Italy.ORCID 0000-0002-9607-229X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The rise of antibiotic resistance underscores the urgent need for new antimicrobial agents. Antimicrobial peptides (AMPs), such as temporins, offer broad-spectrum activity through unique mechanisms but are often limited by cytotoxicity and poor stability. To improve the Gram-negative activity-to-toxicity ratio, we designed a focused library of cyclic Temporin L (TL) analogues bearing an additional positively charged guanidino group, introduced either as a side-chain pendant or as a bridged functionality. While guanidino modifications have been studied in side-chain peptide contexts, this represents the first application of guanidino-based bridging in AMP design. Among the synthesized compounds, four (

Indexed as

Anti-Bacterial AgentsAntimicrobial Cationic PeptidesAntimicrobial PeptidesGram-Negative BacteriaPeptides, CyclicProteinsHumansKeratinocytesMicrobial Sensitivity TestsStructure-Activity RelationshipAnti-Bacterial AgentsAntimicrobial Cationic PeptidesAntimicrobial PeptidesPeptides, CyclicProteinstemporin

Identifiers

PMID41326206
PMCPMC12703733

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.