Evidence map›Paper›PMID 41325030›Full record

ArticleActa crystallographica. Section F, Structural biology communications2026

Crystal structure of Crocus vernus class IIIb chitinase: in silico ligand-binding studies and in vitro antifungal assay.

Ahsan Saeed, Binish Khaliq, Sadaf Iqbal, Sohaib Mehmood, Christian Betzel, Ahmed Akrem

Abstract read
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In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

6 authors.

Ahsan SaeedThe Hamburg Centre for Ultrafast Imaging, Laboratory for Structural Biology of Infection and Inflammation, c/o DESY, University of Hamburg, Building 22a, Notkestrasse 85, 22607 Hamburg, Germany.ORCID 0000-0002-7820-6311
Binish KhaliqDepartment of Botany, University of Okara, Okara, Pakistan.
Sadaf IqbalDepartment of Chemistry, University of Karachi, Karachi, Pakistan.
Sohaib MehmoodThe Hamburg Centre for Ultrafast Imaging, Laboratory for Structural Biology of Infection and Inflammation, c/o DESY, University of Hamburg, Building 22a, Notkestrasse 85, 22607 Hamburg, Germany.
Christian BetzelThe Hamburg Centre for Ultrafast Imaging, Laboratory for Structural Biology of Infection and Inflammation, c/o DESY, University of Hamburg, Building 22a, Notkestrasse 85, 22607 Hamburg, Germany.
Ahmed AkremThe Hamburg Centre for Ultrafast Imaging, Laboratory for Structural Biology of Infection and Inflammation, c/o DESY, University of Hamburg, Building 22a, Notkestrasse 85, 22607 Hamburg, Germany.

Funding

Deutsche Forschungsgemeinschaft 390715994
6 · The paper itself

Abstract

Plant chitinases are found in different organs such as stems, seeds, flowers, corms, tubers and bulbs. In this study, we report the crystal structure of the class IIIb chitinase from Crocus vernus (L.) Hill corms and a thorough comparative analysis with other plant chitinases, especially focusing on molecular-docking interactions between the protein and ligands (allosamidin and chitin oligomer). The C. vernus chitinase (CvChi; PDB entry 3sim) structure has been refined to a crystallographic R factor of 15.5% at a resolution of 2.1 Å. The asymmetric unit is comprised of two chains with 550 residues and 406 water molecules. CvChi has a (β/α)

Indexed as

AcetylglucosamineAntifungal AgentsChitinasesPlant ProteinsAmino Acid SequenceBinding SitesCatalytic DomainChitinCloning, MolecularCrystallography, X-RayGene ExpressionLigandsMolecular Docking SimulationProtein BindingRecombinant ProteinsTrisaccharidesAcetylglucosamineallosamidinAntifungal AgentsChitinchitinase, class IIIChitinasesLigandsPlant ProteinsRecombinant ProteinsTrisaccharideschitinasesCrocus vernuscrystal structureendochitinase activityTIM barrel

Identifiers

PMID41325030
PMCPMC12809453

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.