Evidence map›Paper›PMID 41324882›Full record

ArticleScience China. Life sciences2026

Three-dimensional visualization of tip-vesicles in growing pollen tubes by electron tomography.

Zhiqi Liu, Mengfei Liao, Zizhen Liang, Jiayang Gao, Yixin Huang, Yun Xiang, Tao Ni, Philipp S Erdmann, Liwen Jiang

Abstract read
PubMed Publisher
In one paragraph

Article in Science China. Life sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Structural and Functional Characterization of EXPO-Derived Extracellular Vesicles in Plants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zhiqi Liu *School of Life Sciences, State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, 999077, China.
Mengfei Liao *School of Life Sciences, State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, 999077, China.
Zizhen Liang *School of Life Sciences, State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, 999077, China.
Jiayang GaoSchool of Life Sciences, State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, 999077, China.
Yixin HuangSchool of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 999077, China.
Yun XiangMOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, 730000, China.
Tao NiSchool of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 999077, China.
Philipp S ErdmannHuman Technopole, Milan, 20157, Italy.
Liwen JiangSchool of Life Sciences, State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, 999077, China. ljiang@cuhk.edu.hk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The life cycle of flowering plants starts when a zygote is formed following a double fertilization event. To achieve fertilization, sperm cells are delivered to the female gametes within the embryo sac by the tip-growing pollen tube. The fast-growing pollen tube is characterized and regulated by abundant transport vesicles responsible for both exocytosis and endocytosis in the tip region. Visualization of these tip-vesicles has been challenging owing to their small size, high dynamics, and complexity. In this study, we illustrated the three-dimensional (3D) ultrastructure of tip-vesicles in growing pollen tubes of lily, tobacco, and Arabidopsis. Five major types of tip-vesicles, including secretory vesicles (SVs), electron-dense vesicles (DVs), clathrin-coated vesicles (CCVs), mini vesicles (MVs), and extracellular vesicles (EVs), can be distinguished using room-temperature electron tomography (RT-ET) based on their ultrastructural features. We also demonstrated the extensive distribution of tubular endoplasmic reticulum (ER) structures at the apex of growing pollen tubes and vesicles budding from the tip-localized ER. Cryo-ET further revealed the tip-localized tubular ER with budding coat protein complex II (COPII) vesicles. Our study thus offered a structural basis for a deeper comprehension of vesicular trafficking in the tip growth of the pollen tube, aiding future research on vesicle-mediated membrane trafficking in polarized cell growth.

Indexed as

Electron Microscope TomographyImaging, Three-DimensionalPollen TubeArabidopsisEndoplasmic ReticulumLiliumNicotianaSecretory Vesiclescryo-ETelectron tomographypollen tubetip-vesicle

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.