Evidence map›Paper›PMID 41324174›Full record

ArticleACS nano2025

De Novo Design of α-helical Peptide Nanopores for Single-Molecule Detection Using Helix Packing Motifs.

Zugui Peng, Masataka Usami, Ayaka Nakada, Yusuke Sekiya, Shoko Fujita, Batsaikhan Mijiddorj, Izuru Kawamura, Ryuji Kawano

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS nano, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. bioRxiv : the preprint server for biology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Zugui PengDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo 183-8538, Japan.
Masataka UsamiDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo 183-8538, Japan.
Ayaka NakadaDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo 183-8538, Japan.
Yusuke SekiyaDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo 183-8538, Japan.
Shoko FujitaDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo 183-8538, Japan.
Batsaikhan MijiddorjSchool of Arts and Sciences, National University of Mongolia, Ulaanbaatar 14200, Mongolia.
Izuru KawamuraGraduate School of Engineering Science, Yokohama National University, Yokohama 240-0067, Japan.
Ryuji KawanoDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo 183-8538, Japan.ORCID 0000-0001-6523-0649

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nanopore sensing using transmembrane proteins holds great potential for single-molecule detection. The creation of pore-forming proteins is an emerging research direction, and one promising strategy is the bottom-up de novo design of nanopores. However, de novo α-helical nanopores with sufficiently large dimensions to accommodate single-molecule sensing have not been previously reported. Here, we designed and constructed α-helical nanopores based on two helix-packing motifs, GX

Indexed as

NanoporesPeptidesSingle Molecule ImagingAmino Acid MotifsProtein Conformation, alpha-HelicalPeptidesde novo modelingelectrical conductivitynanoporepeptides and proteinssingle-molecule detection

Identifiers

PMID41324174
PMCPMC12713772

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.