Application of plasma cell-free metagenomic next-generation sequencing for the identification of Aspergillus fumigatus donor-derived infections among solid organ transplant recipients.

Jordan K Mah, John I Hogan, Sonya Kothadia, Jeffrey E Keenan, Johnathan Berger, Manuela Carugati

Abstract readCase Reports

In one paragraph

Article in Medical mycology case reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

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5 · Who and what money

Authors and funding

6 authors.

Jordan K MahDivision of Infectious Diseases, Department of Medicine, Duke University, Durham, 27710, NC, USA.

John I HoganDivision of Infectious Diseases, Department of Medicine, NYU Grossman Long Island School of Medicine, Mineola, 11501, NY, USA.

Sonya KothadiaDivision of Infectious Diseases, Department of Medicine, Duke University, Durham, 27710, NC, USA.

Jeffrey E KeenanDivision of Cardiovascular and Thoracic Surgery, Department of Surgery, Duke University, Durham, 27710, NC, USA.

Johnathan BergerTransplant Institute, NYU Grossman Long Island School of Medicine, Mineola, 11501, NY, USA.

Manuela CarugatiDivision of Infectious Diseases, Department of Medicine, Duke University, Durham, 27710, NC, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A cluster of Aspergillus fumigatus donor-derived infections (DDI) was rapidly diagnosed using plasma metagenomic next-generation sequencing (mNGS) among solid organ transplant recipients. The heart recipient, experiencing marginal hemodynamics, underwent an endomyocardial biopsy, which was concerning for a fungal infection on histopathology. Plasma mNGS was performed, identifying A. fumigatus two days prior to conventional diagnostics. This timely diagnosis enabled prompt nephrectomies in the kidney recipients, who survived. This report represents the first published use of mNGS in the diagnosis of Aspergillus fumigatus DDI, highlighting the utility of this novel, underutilized assay for early diagnosis of donor-derived infections.

Indexed as

Aspergillus sppDonor-derived infectionsInvasive fungal infectionMetagenomic next-generation sequencingSolid organ transplantation

Identifiers

PMID41324077

DOI10.1016/j.mmcr.2025.100751

PMCPMC12663023

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