ArticleMechanobiology in medicine2025
A practical protocol of processing mineralized tissue for Visium spatial transcriptomics.
Article in Mechanobiology in medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Neuroimmune Regulation in Posttraumatic Bone Repair: From Inflammatory Transition to Neurovascular Coupling and Functional Recovery.Journal of cellular physiology · 2026Review
- Stage-resolved geography of mouse skeletal stem cells.Journal of bone and mineral metabolism · 2026Review
- Spatial multi-omics decoding of the fracture nonunion niche: from immune-vascular-skeletal crosstalk to precision regeneration.Frontiers in medicine · 2026Review
- Bone formation niche dysfunction in osteoporosis: insights from single-cell and spatial transcriptomic studies.Frontiers in endocrinology · 2026Review
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Authors and funding
5 authors.
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Abstract
Spatial transcriptomics analysis of mineralized tissues faces significant challenges due to lengthy decalcification procedures that severely compromise RNA integrity and subsequent gene detection. This protocol details an optimized workflow to process bone and fracture callus samples for 10x Genomics Visium spatial transcriptomics. The key innovation involves replacing conventional EDTA decalcification with Morse's solution, enabling rapid decalcification (<24 h) while preserving RNA quality, as evidenced by a favorable DV200 value. Additionally, the protocol emphasizes the critical use of SCHOTT NEXTERION® Slide H (3-D hydrogel-coated) to maximize adherence of fragile decalcified bone sections during processing, preventing detachment and preserving morphological integrity. We applied this optimized method to intact and fractured mouse femurs. The results demonstrate a substantial improvement in transcript capture efficiency: Visium V2 yielded an average of 5639 genes per spot, while Visium HD achieved an average of 170 genes per 8 μm bin (equivalent to ∼4100 genes
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