ArticleiScience2025
Rapid neonatal AAV delivery for adult cortical two-photon imaging of genetically encoded sensors.
Article in iScience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Ketamine persists within neuronal compartments long after systemic clearance.Research square · 2026Article
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7 authors.
Funding
Abstract
Elucidating the mechanisms of the central nervous system necessitates the ability to precisely visualize, record, and manipulate specific neuronal populations within the living brain. Here, we report a fast and efficient technique for delivering adeno-associated virus (AAV) in neonatal pups for the robust co-expression of reporters and sensors in cortical neuronal subpopulations under different promoters and transgenic mouse lines. This approach has lower training requirements than adult AAV injections, and it enables a 40- to 50-fold increase in injection throughput per animal with high reproducibility. Pup-injected adult mice show reduced signals of neuroinflammation compared to adult-injected mice. Importantly, adult pup-injected mice have stable and high expression of multiple AAVs suitable for structure-function two-photon imaging in local cortical circuits of awake, behaving adult animals. This approach offers a fast, efficient, and robust method of introducing multiple AAVs for investigating neuronal structure and function in defined cortical neuronal populations in the living brain.
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