ArticleMethodsX2025
Isolation, identification and optimization of biomass production of native Spirulina species from South of the Caspian Sea.
Article in MethodsX, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The aim of the present study was to identify the native Spirulina species from Caspian Sea and optimize the growth conditions of this microalgae in order to reduce costs and increase the benefits of its production. In this study, water sampling was carried out from a depth of 5 meters on the coast of south Caspian Sea (Iran) in 2023-2024. After observing the Spirulina genus, its microalgae were isolated and purified using the dilution method, and finally pure single colonies were obtained. Initial identification was carried out using a light microscope and valid identification keys. Molecular identification and 16 SrRNA sequencing were used to confirm and verify it. The results showed that the studied species has the least genetic distance from the microalgae
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