Preparation, physicochemical properties, and immunomodulatory activity of glycoproteins from Syngnathoides biaculeatus.

Xuewei Xia, Jieling Lin, Lihong Yang, Youhong Li, Wenshen Lin, Qingqing Wang, Jun Liu, Riming Huang

Abstract read

In one paragraph

Article in Natural products and bioprospecting, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Xuewei XiaGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.

Jieling LinGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.

Lihong YangGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.

Youhong LiGuangzhou University of Chinese Medicine, Guangzhou, 510006, China.

Wenshen LinGuangzhou University of Chinese Medicine, Guangzhou, 510006, China.

Qingqing WangGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.

Jun LiuLaboratory of Pathogenic Biology, Guangdong Medical University, Zhanjiang, 524023, China. lj2388240@gdmu.edu.cn.

Riming HuangGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China. huangriming@scau.edu.cn.

Funding

Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety GDPKLAPPS2102Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety GDPKLAPPS2304Key-Area Research and Development Program of Guangdong Province 2022B0202020004Medical Scientific Research Foundation of Guangdong Province A2025237Shenzhen Science and Technology Program KCXFZ20240903094014019

6 · The paper itself

Abstract

Syngnathoides biaculeatus, a potential functional food from marine sources, was found to enhance nonspecific immunity, but its functional ingredients have rarely been reported. Therefore, this study focused on the preparation and physicochemical properties of its water-soluble natural ingredients with their immunomodulatory activities and potential mechanisms. Firstly, we optimized the extraction method of glycoproteins from S. biaculeatus and prepared the crude glycoprotein SYB, from which the fraction glycoprotein SYB-1 was further purified. The carbohydrates of SYB-1 were 8.46% comprising mannose, glucose, and galactose, with an average molecular weight of 9.423 kDa. Amino acid analysis demonstrated that its major amino acids are glycine, glutamic acid, aspartic acid, and proline, with a total amino acid content of 88.81%. Furthermore, SYB-1 could significantly increase the cell viability of macrophages, and promote the release of NO, TNF-α, and IL-6. Metabolomics revealed that it was associated with arachidonic acid metabolism. The CYP450 enzyme family members and PTGS2 may be key targets for the regulatory role. These results suggested that the glycoprotein of S. biaculeatus may be an attractive functional food supplement from natural sources for immunocompromised populations.

Indexed as

GlycoproteinImmunomodulatory activityPhysicochemical propertiesSyngnathoides biaculeatus

Identifiers

PMID41320746

DOI10.1007/s13659-025-00551-6

PMCPMC12665643

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