ArticleBMC cancer2025
Comprehensive long-read transcriptomic analysis reveals multi-level transcriptional alterations mediated by miR-214-3p dysregulation in gastric cancer cells.
Article in BMC cancer, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundGastric cancer remains one of the leading causes of cancer-related death worldwide. miR-214-3p, a microRNA, has been reported to exhibit dysregulated expression and play important regulatory roles in various cancers. However, the global targets and underlying mechanisms of miR-214-3p in gastric cancer progression remain poorly understood.
methodsIn this study, we performed long-read transcriptomic sequencing to comprehensively characterize transcriptome-wide variations in AGS gastric cancer cells following miR-214-3p overexpression (OE) or knockdown (KD). Functional in vitro experiments were conducted to validate the newly identified transcripts and assess the effects of miR-214-3p OE on cell proliferation and apoptosis in AGS cells.
resultsOur results demonstrated that miR-214-3p OE and KD significantly altered the expression levels of numerous genes at both transcript and gene levels, particularly those involved in apoptosis and transcriptional regulation. Interestingly, miR-214-3p KD and OE showed consistent directional effects on the expression of some target genes. Additionally, miR-214-3p broadly influenced the alternative splicing of hundreds of genes, including those associated with cell division, senescence, and transcriptional regulation. Notably, changes in miR-214-3p expression also affected the alternative polyadenylation and 3’UTR lengths of transcripts. Furthermore, our in vitro experiments revealed that miR-214-3p OE significantly promoted the proliferation of AGS cells and inhibited apoptosis, suggesting its potential oncogenic role.
conclusionsCollectively, these findings highlight the potential multifaceted regulatory roles of miR-214-3p in mediating proliferation and apoptosis through diverse mechanisms in AGS gastric cancer cells, thereby advancing our understanding of its critical role in gastric cancer progression and its therapeutic potential.
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