ArticleBMC plant biology2025
Genomic insights and SSR marker development for trait improvement in Pleurotus giganteus for tropical cultivation.
Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
backgroundPleurotus giganteus is a medium-high temperature mushroom crop with strong potential for commercial cultivation in tropical regions. Understanding its genomic and genetic diversity is key to developing improved germplasm.
resultsIn this study, we performed de novo genome sequencing of strain PG79, which exhibits fast growth and desirable morphology but is sensitive to heat and disease, and compared it with PG46, a heat-tolerant, disease-resistant strain with slower growth and long stipes. Both genomes were similar in size (PG79: 40.46 Mb; PG46: 40.11 Mb) but differed in non-coding gene content, with PG79 containing more rRNA and snRNA genes. PG79 harbored more genes related to substrate utilization, growth and development, and secondary metabolism, such as GH5, terpene, T1PKS, and NRPS-like genes. The genomes shared high synteny (84%) with limited structural variation. Molecular clock analysis estimated divergence at ~ 8.6 million years ago, with PG79 showing gene family expansions related to signal transduction, biosynthesis, and recombination. Population genomic analysis of 32 strains revealed clear differentiation among wild, cultivated, and hybrid groups, though cultivated strains exhibited reduced genetic diversity. From the PG46 genome, 16, 5, and 7 novel SSR markers were developed for monokaryon identification, mating type differentiation, and hybrid verification, respectively. PG46 and PG79, with complementary traits and distinct genetic backgrounds, were used as breeding parents. Among hybrids, PGH6 showed excellent agronomic performance, including heat tolerance, compact morphology, short growth cycle, and high nutritional value.
conclusionOur study successfully obtained an improved Pleurotus giganteus strain by combining genomic analysis with marker-assisted breeding. This study provided valuable genomic resources and a marker-assisted breeding framework to accelerate the improvement of P. giganteus for tropical mushroom cultivation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.